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目的研究天然化合物青藤碱对人前列腺癌DU145细胞增殖及凋亡的影响。方法采用MTT法检测青藤碱对DU145细胞增殖的影响;采用流式细胞仪AnnexinV/PI双染法检测青藤碱对DU145细胞凋亡的影响;采用电子显微镜观察青藤碱作用DU145细胞后细胞超微结构的变化。结果 MTT结果显示青藤碱浓度在100～400μmol/L时,作用24、48小时后,细胞增殖受到抑制,呈现明显时间剂量效应关系;流式细胞仪AnnexinV/PI双染法显示青藤碱(0、200、400μmol/L)作用于细胞24小时后,细胞凋亡率升高,且呈剂量效应关系;电子显微镜下青藤碱处理组细胞可见染色质浓聚、边集等典型的凋亡改变,而空白对照组没有相应的变化。结论青藤碱能有效抑制人前列腺癌DU145细胞增殖、诱导细胞凋亡。
Objective To study the effect of sinomenine, a natural compound, on the proliferation and apoptosis of human prostate cancer DU145 cells. Methods The effect of sinomenine on the proliferation of DU145 cells was detected by MTT assay. The effect of sinomenine on the apoptosis of DU145 cells was detected by flow cytometry Annexin V / PI double staining. The effect of sinomenine on DU145 cells was observed by electron microscopy Changes in ultrastructure. Results The results of MTT showed that when the concentration of sinomenine was in the range of 100-400 μmol / L, the cell proliferation was inhibited after 24 and 48 hours, and the time-dose-effect relationship was observed. The results of flow cytometry Annexin V / PI double staining showed that sinomenine 0, 200, 400μmol / L) for 24 hours, the cell apoptosis rate increased, and the dose-response relationship; under electron microscopy sinomenine treatment group of chromatin condensation, edge set and other typical apoptosis Change, while the blank control group did not change accordingly. Conclusion Sinomenine can effectively inhibit the proliferation of human prostate cancer DU145 cells and induce apoptosis.