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本文介绍以反相薄层层析法和红外光谱法分离测定利福霉素O和利福霉素S。实验采用硅胶G(Merck)薄层板,将预涂板浸入尿素的无水乙醇饱和溶液中约60秒取出,温热后使用。对照标准用氯仿溶液制备,样品中含利福霉素O和利福霉素S的比值可高达1:10到1:100。点样后,将层析板放入用乙醚饱和2小时的层析缸中,乙醚作流动相,展层10cm后取出,即用氨蒸气显色,利福霉素S显紫色。利福霉素O的红外光谱在1820cm处有吸收,是由于五节杂环上的羰基振动所致,用Perkin Elmer 180
This article describes the separation and determination of rifamycin O and rifamycin S by reversed-phase thin-layer chromatography and infrared spectroscopy. Experiments using silica gel G (Merck) TLC, the pre-coated plate immersed in urea saturated ethanol solution about 60 seconds to remove, warm use. Control standards prepared with chloroform solution, the sample containing rifamycin O and rifamycin S ratio can be as high as 1:10 to 1:100. After spotting, the chromatographic plate was placed in a chromatography cylinder saturated with diethyl ether for 2 hours. Ether was used as the mobile phase and the layer was taken out after 10 cm. That is, the color was developed with ammonia vapor, and rifamycin S was purple. The infra-red spectrum of rifamycin O is absorbed at 1820 cm due to the carbonyl vibration on the five-membered heterocycles, which is analyzed by Perkin Elmer 180