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构建恶性疟原虫FCC1／HN株MSP1基因2～5编码区真核表达质粒pcDNA3／MSP1，为FCC1／HN株MSP1的体外表达及其基因工程疫苗的研制奠定基础。方法采用PCR技术对恶性疟原虫海南分离株（FCC1／HN）基因组DNAMSP1第2～5区基因片段进行扩增．扩增产物经纯化后用Xhol和Aaal双酶切然后定向克隆入pcDNA3质粒，转化大肠杆菌TG1．再用相同内切酶酶切和PCR扩增对重组子进行鉴定。结果筛选出编码FCC1／HN株MSP1第2～5区基因片段的真核表达质粒pcDNA3／MSP1。结论编码FCC1／HN株MSPI第2～5区基因片段的真核表达质粒pcDNA3／MSP1的构建，为恶性疟原虫FCC1／HN株MSP1的体外表达及其基因工程疫苗的研制奠定基础。
To construct eukaryotic expression plasmid pcDNA3 / MSP1 of MSP1 gene 2 ~ 5 of Plasmodium falciparum FCC1 / HN strain, and to lay the foundation for the in vitro expression of MSP1 and the development of gene engineering vaccine of FCC1 / HN strain. Methods The PCR products were used to amplify the 2 ~ 5 region of MSP1 gene from Plasmodium falciparum Hainan isolate (FCC1 / HN). The amplified product was purified and double-digested with Xhol and Aal and then cloned into the pcDNA3 plasmid for transformation into E. coli TG1. The recombinants were identified by restriction endonuclease digestion and PCR amplification. Results The eukaryotic expression plasmid pcDNA3 / MSP1 encoding the 2 ~ 5 region of MSP1 gene of FCC1 / HN strain was screened out. Conclusion The construction of eukaryotic expression plasmid pcDNA3 / MSP1 encoding the gene fragment of MSPI region 2 ~ 5 of FCC1 / HN strain laid the foundation for the in vitro expression of MSP1 of Plasmodium falciparum FCC1 / HN strain MSP1 and its genetic engineering vaccine.