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目的:观察微波照射对兔肝脏缺血再灌注损伤中的肝功能ALT、A ST、LDH、肝组织匀浆一氧化氮(NO)、一氧化氮合酶(NO S)的变化,并探讨其保护机制。方法:32只新西兰大白兔被随机分为A、B、C、D共4组。各组于术后2h、4 h抽血查ALT、A ST、LDH。实验结束处死获取肝脏标本,检测各组的NO、NO S并行HE染色。结果:B、C、D三组的ALT、A ST、LDH显著高于A组。而C、D组的NO、NO S显著低于A、B两组,其中D组又显著低于C组。C组病理改变明显轻于D组。结论:适宜条件的微波照射预处理能明显改善缺血再灌注损伤的肝功能,减轻肝细胞损伤,而NO是该保护机制中的一个重要因素。
Objective: To observe the changes of hepatic function ALT, AST, LDH, hepatic homogenate nitric oxide (NO) and nitric oxide synthase (NO S) in rabbit liver after ischemia-reperfusion injury protection mechanism. Methods: 32 New Zealand white rabbits were randomly divided into A, B, C, D 4 groups. Blood samples were collected at 2h and 4h after operation for ALT, AST and LDH. At the end of the experiment, the liver specimens were sacrificed and the NO and NO S in each group were detected by HE staining. Results: The ALT, A ST, LDH of group B, C and D were significantly higher than that of group A. However, NO and NO S in group C and group D were significantly lower than those in group A and group B, and group D was significantly lower than group C. C group pathological changes were significantly lighter than the D group. CONCLUSION: Pretreatment with microwave irradiation under appropriate conditions can significantly improve the liver function of ischemia-reperfusion injury and reduce the damage of hepatocytes. However, NO is an important factor in this protective mechanism.