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虫害是杨树造林生产中所面临的一大难题。通过农杆菌介导 ,将经过人工改造的苏云金杆菌杀虫结晶蛋白 (Bt)基因转化杨树 ,得到杨树再生植株 ,然后 ,再将蛋白酶抑制剂基因 (PI)导入已含Bt基因的转基因杨树 ,经PCR检测和Southern杂交分析证明 ,最终获得既含有Bt基因又含有蛋白酶抑制剂基因的转基因杨树植株。利用这种杨树叶片饲喂舞毒蛾幼虫的杀虫试验结果表明 ,转基因杨树具有明显的杀虫活性 ,同时表明含有Bt基因和蛋白酶抑制剂基因双基因的植株其抗虫能力明显高于仅含单一Bt基因的植株。为探索培育高抗虫性杨树开辟了一条新途径。
Pests are a big challenge in poplar afforestation production. Through Agrobacterium-mediated transformation, the genetically modified Bacillus thuringiensis insecticidal crystal protein (Bt) gene was transformed into poplar to obtain poplar regeneration plants, and then the protease inhibitor gene (PI) was introduced into transgenic poplar with Bt gene The results of PCR and Southern blot showed that transgenic poplar plants containing both Bt and protease inhibitors were obtained. The insecticidal test results of feeding poppy moth larvae on the poplar leaves showed that the poplar has obvious insecticidal activity and the insect resistance ability of plants containing the double gene of Bt gene and protease inhibitor gene was significantly higher than that of the only Plants with a single Bt gene. In order to explore the cultivation of poplar high insect resistance has opened up a new way.