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目的了解十堰市霍乱弧菌污染状况,为疫情分析,预警及制定防控策略提供科学依据。方法采集市场销售的生畜禽肉、水产品、外环境水体、苍蝇、牛粪等,按《霍乱防治手册》进行检验。结果共采集样品52份,分别从基围虾和生猪肉中各检出1株小川型霍乱弧菌,阳性率为3.84%。这两株菌ctxA毒力基因测检阴性为非流行株,药敏试验表明它们对头孢噻肟、氯霉素、四环素敏感。结论十堰市生畜肉类,水产品中存在霍乱弧菌污染状况,虽经鉴定为无毒力非流行株,但仍需加强监测和消毒力度,防止疾病和疫情的发生。
Objective To understand the status of Vibrio cholerae in Shiyan City and provide a scientific basis for epidemic situation analysis, early warning and formulation of prevention and control strategies. Methods Live and poultry meat, aquatic products, external environmental water bodies, flies and cow manure collected from the market were tested according to “Cholera Prevention and Treatment Manual”. Results A total of 52 samples were collected. One strain of V. cholerae was detected from the shrimp and pork, respectively, with a positive rate of 3.84%. The two strains of ctxA virulence negative genetic test for non-epidemic strains, susceptibility testing showed that they are sensitive to cefotaxime, chloramphenicol, tetracycline. Conclusion There is Vibrio cholerae pollution in livestock and meat products and aquatic products in Shiyan City. Although it has been identified as non-virulent non-epidemic strain, monitoring and disinfection should be strengthened to prevent diseases and outbreaks.