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目的:研究中药复方“松友饮”的体内药效物质基础并重新配伍设计,为其组方优化提供理论基础。方法:应用高转移人肝细胞癌细胞株MHCC97H构建裸鼠原位移植瘤模型,24只成模裸鼠随机平均分成对照组和“松友饮”组,肝脏接种肿瘤1 d开始1次/d灌胃给药(“松友饮”3.6 g/kg,对照组予以等量蒸馏水)。灌胃5周,脱颈处死裸鼠收集血样,用于进一步分析“松友饮”体内药效物质基础,采用均匀设计的实验方法重新设计、配伍、优化“松友饮”。结果:裸鼠模型构建过程顺利,灌胃过程中实验组死亡1只裸鼠,有效获取含药血清。结合参考文献及通过比对发现连续给药5周,“松友饮”吸收入血的主峰来自组方药材成分:黄芪甲苷、槲皮素及隐丹参酮、丹参酮IIA(新组复方主要含4种单体,简称HHYD复方),重新配伍为3种新组方。结论:基于5周给药的含药血清药效物质基础新组方的组分更加明确,更易定量和质量控制。
OBJECTIVE: To study the pharmacodynamic basis of traditional Chinese medicine compound “Song Youyin” and to redesign the compatibility, which provides a theoretical basis for the optimization of the prescriptions. Methods: The nude mice transplantation tumor model was established by using highly metastatic human hepatocellular carcinoma cell line MHCC97H. Twenty-four nude mice were randomly divided into control group and “Songyouyin” group. The liver tumor inoculation was started 1 day / d gavage ( “Song Friends drink ” 3.6 g / kg, the control group to be equal to distilled water). Five weeks after gavage, the nude mice were sacrificed and the blood samples were collected for further analysis of the substance basis of “Song Youyin” in vivo. The redesigned, compatibilized and optimized “Songyouyin” was experimentally designed with uniform design. Results: The nude mouse model was successfully constructed. One nude mouse died in the experimental group during gavage, and the drug-containing serum was obtained effectively. Combined with reference and comparison found that continuous administration for 5 weeks, “Song Youyin ” absorbed into the blood of the main peak from the prescription ingredients: Astragaloside, quercetin and cryptotanshinone, tanshinone IIA 4 kinds of monomer, referred to as HHYD compound), re-compatibility of three new groups. CONCLUSIONS: Components based on 5-week drug-containing serum-based pharmacological agents are more specific, more quantitative and quality-controlled.