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目的探讨黑榆根皮有效成分表木栓醇抑制UVB照射引起的皮肤老化作用及机制。方法在37°C、5%CO_2、95%空气饱和湿度的培养箱内体外培养原代人真皮成纤维细胞,将细胞分为5组:即正常对照组、UVB照射模型组、表木栓醇30μg·ml~(-1)组、表木栓醇50μg·ml~(-1)组、表木栓醇100μg·ml~(-1)组,研究表木栓醇对模型照射引起人真皮成纤维细胞老化的作用以及对老化调控蛋白p53表达的影响。在裸鼠背部皮肤上分成了4组,即正常对照组、模型组、表木栓醇20μg·g-1组、表木栓醇50μg·g-1组,研究表木栓醇在体内真皮层的药效作用。结果细胞实验显示,表木栓醇浓度依赖性地抑制UVB照射引起的人真皮成纤维细胞的老化,浓度依赖性地抑制老化调控蛋白p53的表达。模型组与正常对照组比较,SA-β-gal染色率显著升高,p53表达显著升高,表木栓醇50,100μg·ml~(-1)组与模型组比较,SA-β-gal染色率都显著降低(P<0.05,P<0.01),p53表达也都显著降低。动物实验显示,模型组与正常对照组比较,真皮层SA-β-gal染色明显增强,表木栓醇50μg·g-1组与模型组比较,真皮层SA-β-gal染色明显减弱。结论黑榆根皮成分表木栓醇通过调控p53的表达抑制UVB照射引起的皮肤老化。
Objective To investigate the effect and mechanism of epimiramycin on skin aging induced by UVB irradiation. Methods Primary human dermal fibroblasts were cultured in vitro at 37 ° C, 5% CO 2 and 95% air saturated humidity. The cells were divided into 5 groups: normal control group, UVB irradiation model group, 30μg · ml ~ (-1) group, epiclianoide 50μg · ml ~ (-1) group, epiclostanol 100μg · ml ~ (-1) group. The effect of fibroblast aging and its effect on the expression of aging regulatory protein p53. The nude mice were divided into four groups on the back skin: normal control group, model group, epiclodextrin 20μg · g-1 group, epiclosporin 50μg · g-1 group. Efficacy effect. Results Cell experiments showed that epicatechin inhibited the aging of human dermal fibroblasts in a concentration-dependent manner and inhibited the expression of aging regulatory protein p53 in a concentration-dependent manner. Compared with the normal control group, the staining rate of SA-β-gal and the expression of p53 in the model group were significantly increased. The expression of SA-β-gal in 50, 100μg · ml ~ (-1) The rates were significantly lower (P <0.05, P <0.01), p53 expression were also significantly reduced. Animal experiments showed that the SA-β-gal staining of the dermis was significantly increased in the model group compared with the normal control group. The SA-β-gal staining in the dermis was significantly weaker in the 50 μg · g-1 epicatechin group compared with the model group. Conclusion Cinnamomum cassia alcohol inhibits skin aging induced by UVB by regulating the expression of p53.