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作者采用荧光原位杂交技术(FISH),对市售的着丝粒DNA探针与未培养的羊水细胞原位杂交诊断染色体非整倍性的可靠性进行了评估。 选择116例孕3～30周高龄及超声诊断胎儿异常的孕妇,取羊水15～20ml,其中13～18ml羊水培养用于标准核型分析。其余2～5ml羊水直接进行荧光原位杂交。选用生物
The authors used fluorescence in situ hybridization (FISH) to assess the reliability of diagnostic chromosomal aneuploidy by in situ hybridization of commercially available centromere DNA probes and uncultured amniotic fluid cells. One hundred and sixty-six pregnant women with gestational age from 3 to 30 weeks and ultrasound to diagnose fetal abnormalities were selected. Fifteen to twenty ml of amniotic fluid were selected. Among them, 13-18 ml of amniotic fluid were used for standard karyotype analysis. The remaining 2 ~ 5ml amniotic fluid directly fluorescence in situ hybridization. Use biological