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作者通过逆转录和PCR扩增获得cDNA,克隆后表达为具有谷胱苷肽S-转移酶(GST)性质的融合蛋白,并用ELISA对多房棘球绦虫和细粒棘球绦虫的全长抗原Ⅱ/3进行比较分析。 按Glisin等(1974)和Ullrich等(1977)的改良方法进行总RNA纯化。分别取3g多房棘球蚴组织和0.4g细粒棘球蚴育囊,在5
The cDNA was obtained by reverse transcription and PCR amplification. The cDNA was cloned and expressed as a fusion protein with glutathione S-transferase (GST). The full-length antigen of Echinococcus granulosus and Echinococcus granulosus Ⅱ / 3 for comparative analysis. Total RNA purification was performed according to the modified method of Glisin et al. (1974) and Ullrich et al. (1977). Respectively take 3g Echinococcus multilocularis tissue and 0.4g Echinococcus granulosus cyst at 5