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目的:建立同时测定茵陈蒿中绿原酸、咖啡酸和对羟基苯乙酮含量的方法。方法:采用紫外-双波长同时检测的RP-HPLC法:C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.04%磷酸水溶液(9∶91),流速1.0mL·min-1,检测波长:325nm(绿原酸、咖啡酸);275nm(对羟基苯乙酮)。结果:绿原酸、咖啡酸和对羟基苯乙酮进样浓度分别在52.10～1.303×103μg·mL-1(r=0.9999)、1.756～43.91μg·mL-1(r=0.9999)、0.6574～16.43μg·mL-1(r=0.9995)范围内与峰面积呈良好的线性关系(n=5);方法回收率(n=9)分别为98.1%,98.6%,100.8%。结论:方法简便、快速、准确,重现性好,为茵陈蒿药材的质量控制提供依据。
Objective: To establish a method for simultaneous determination of chlorogenic acid, caffeic acid and p-hydroxy acetophenone in Artemisia capillaris. Methods: RP-HPLC with UV-Dual Wavelength Simultaneous Detection: C18 column (250mm×4.6mm, 5μm), mobile phase of acetonitrile-0.04% phosphoric acid aqueous solution (9:91), flow rate 1.0mL·min-1, detection Wavelength: 325 nm (chlorogenic acid, caffeic acid); 275 nm (p-hydroxyacetophenone). Results: The concentrations of chlorogenic acid, caffeic acid and p-hydroxy acetophenone were 52.10～1.303×103μg·mL-1(r=0.9999),1.756～43.91μg·mL-1(r=0.9999),0.6574～, respectively. There was a good linear relationship with the peak area in the range of 16.43 μg·mL-1 (r=0.9995) (n=5). The recoveries of the method (n=9) were 98.1%, 98.6% and 100.8%, respectively. Conclusion: The method is simple, rapid, accurate, and reproducible. It provides the basis for the quality control of Artemisia annua.