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目的 检测食管鳞癌中的人乳头瘤病毒 (HPV)感染 ,探索HPV与食管癌大体生长方式的关系。方法 应用通用引物PCR法和多重PCR法检测 10 0例食管癌组织标本中的HPV感染。结果 共检测出HPV阳性 2 6例 (2 6 / 10 0 ) ,按照食管癌的病理大体分型 ,其中溃疡型食管癌中HPV阳性 5例 (5 / 2 5 )、蕈伞型中阳性 3例 (3/ 2 5 )、缩窄型中阳性 12例 (12 / 2 5 )、髓质型中阳性 6例 (6 / 2 5 )。对 2 6例HPV阳性标本中的 2 0例进行了多重PCR检测 ,发现HPV6 / 11型 6例、HPV16型 11例、HPV18型 3例。结论 我国食管癌中存在HPV感染 ,存在HPV感染的食管癌可能并不是在HPV引起的湿疣样病变基础上发展而来。
Objective To detect human papillomavirus (HPV) infection in esophageal squamous cell carcinoma (ESCC) and to explore the relationship between HPV and the general growth pattern of esophageal cancer. Methods The universal primer PCR and multiplex PCR were used to detect HPV infection in 100 cases of esophageal cancer tissue samples. Results A total of 26 HPV positive cases (26/10) were detected. According to the pathological classification of esophageal cancer, 5 were HPV positive (5/25), 3 were positive (3/25), 12 (25%) positive in constriction type and 6 (2.5%) positive in medullary type. Multiple PCR tests were performed on 20 of 26 HPV-positive specimens and found 6 cases of HPV type 6/11, 11 cases of HPV type 16 and 3 cases of HPV type 18. Conclusion There is HPV infection in esophageal cancer in our country. Esophageal cancer with HPV infection may not be developed on the basis of genital warts caused by HPV.