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为探讨SUMO(类泛素的小蛋白修饰,small ubiquitin-like modifier)在大豆逆境胁迫中的作用,对大豆SUMO系统的相关基因和蛋白质结构进行分析,与其它植物SUMO化相关基因构建系统发育树,并将大豆合丰25热激处理后,选取SUMO系统中的6个基因(Gm SUMO2,Gm SUMO3,Gm SAE1b,Gm SCEb,Gm E3f,Gm ESD4d)进行实时定量分析。结果表明,大豆Gm SUMO,Gm SAE1,Gm SCE,Gm E3f与木本植物亲缘关系较近;热激处理不同时段,大豆6个SUMO相关基因均有明显变化,Gm SAE1b起激活作用最先启动,Gm SUMO2/3和Gm SCEb表达趋势一致,验证了Gm SCEb的结合作用。热激10min后,Gm SUMO2/SUMO3相对表达量达到最低,而Gm ESD4d在叶中相对表达量达到近40倍,说明Gm ESD4d在叶中起去SUMO化作用。热激30min,在根中Gm SUMO2相对表达量达到近27倍,Gm SCEb达到10倍,Gm E3f达到近40倍。说明Gm SUMO2、Gm SCEb、Gm E3f主要在根中起作用。
In order to investigate the role of SUMO (small ubiquitin-like modifier) in stress response to soybean stress, the related gene and protein structure of soybean SUMO system were analyzed, and the phylogenetic tree of SUMO related genes of other plants was constructed , And heat shock treatment of soybean Hefeng 25, 6 genes (Gm SUMO2, Gm SUMO3, Gm SAE1b, Gm SCEb, Gm E3f, Gm ESD4d) in SUMO system were selected for real-time quantitative analysis. Gm SAE1, Gm SCE and Gm E3f were closely related to woody plants. The results showed that all of the 6 SUMO related genes in soybean had significant changes at different time periods after heat shock treatment, and the activation of Gm SAE1b was the first to start, Gm SUMO2 / 3 and Gm SCEb expression trend consistent, verifying the binding of Gm SCEb. The relative expression of Gm SUMO2 / SUMO3 reached the minimum after heat shock for 10min, while the relative expression level of Gm ESD4d in leaves reached nearly 40 times, indicating that Gm ESD4d played a SUMO role in the leaves. Heat shock 30min, the relative expression of Gm SUMO2 in the root reached nearly 27 times, Gm SCEb reached 10 times, Gm E3f reached nearly 40 times. Gm SUMO2, Gm SCEb, Gm E3f mainly play a role in the root.