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目的:建立液相色谱法测定田七痛经散中三七皂苷R_1及人参皂苷Rg_1、Rb_1含量。方法:采用Watres Sunfire C_(18)(250 mm×4.6 mm,5μm)色谱柱,乙腈-水为流动相,梯度洗脱,流速1.0 ml·min~(-1),检测波长203 nm,柱温30℃。结果:三七皂苷R_1线性范围为0.21～2.1μg(r=0.9999),平均加样回收率为98.8%,RSD为1.51%(n=6);人参皂苷Rg_1线性范围为0.78～7.18μg(r=0.9999),平均加样回收率为99.9%,RSD为1.92%(n=6);人参皂苷Rb_1线性范围为0.81～8.10μg(r=0.9998),平均加样回收率为98.8%,RSD为1.63%(n=6)。结论:方法灵敏、准确,重复性好,可用于制剂的含量测定及质量控制。
Objective: To establish a HPLC method for the determination of Panax notoginseng R_1 and ginsenosides Rg_1, Rb_1 in Tianqi Tongjing. Methods: The mobile phase was eluted with a mobile phase of Watre Sunfire C 18 (250 mm × 4.6 mm, 5 μm) with gradient elution at a flow rate of 1.0 ml · min -1. The detection wavelength was set at 203 nm. The column temperature 30 ° C. Results: The linear range of notoginsenoside R_1 was 0.21 ~ 2.1μg (r = 0.9999), the average recovery was 98.8% with RSD 1.51% (n = 6). The linear range of ginsenoside Rg_1 was 0.78 ~ 7.18μg = 0.9999). The average recovery was 99.9% and the RSD was 1.92% (n = 6). The linear range of ginsenoside Rb_1 was 0.81 ~ 8.10μg (r = 0.9998) 1.63% (n = 6). Conclusion: The method is sensitive, accurate and reproducible. It can be used to determine the content and quality control of the preparation.