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[目的]获取Gm HIR1相关生物信息学信息,分析与其他植物HIR的进化亲缘关系,进行启动子原件和结构的预测,分析其潜在调控功能,并研究该基因在疫霉菌侵染大豆过程中的表达情况。[方法]利用生物信息学软件对GmHIR1进行分析和功能预测,将大豆疫霉菌侵染抗病品种绥农10号和感病品种合丰25,分7个时间点进行取样,对GmHIR1的表达情况进行qRT-PCR分析。[结果]Gm HIR1启动子含有11个作用元件,该基因编码蛋白含有stomatins和prohibitin两个结构域,表达分析表明Gm HIR1在接种疫霉菌36 h之前,在抗病品种绥农10号中表达明显增加,而在感病品种合丰25中变化不明显,36 h之后,在两个品种的表达量变化趋势相似。[结论]Gm HIR1与多种生理反应相关,并且确认Gm HIR1与抗病大豆防御疫霉菌相关。
[Objective] The research aimed to obtain the bioinformatics information of Gm HIR1, analyze the evolutionary genetic relationship with HIR of other plants, predict the promoter and structure of the promoter, analyze its potential regulatory function, and study the influence of the gene on Phytophthora infestans in soybean Express the situation. [Method] The bioinformatics software was used to analyze and predict the function of GmHIR1. The resistant soybean cultivar Suinong 10 and susceptible cultivar Hefeng 25 were infected with Phytophthora sojae at a sampling time of 7 points. The expression of GmHIR1 QRT-PCR analysis was performed. [Result] The Gm HIR1 promoter contained 11 active elements. The gene contained two domains, stomatins and prohibitin. The expression analysis showed that Gm HIR1 was expressed in Suinong 10 before the inoculation of 36 days Increased, but did not change significantly in the susceptible variety Hefeng25. After 36 h, the trend of expression in two varieties was similar. [Conclusion] Gm HIR1 is related to a variety of physiological responses and it is confirmed that Gm HIR1 is associated with disease-resistant soybean defense against Phytophthora capsici.