Cathepsin B antisense oligodeoxynucleotide suppresses invasive potential of MG-63 cells

• 作 者: 贺茂林 肖增明 李世德 陈安民
• 机 构: Department of Spinal Surgery,the First Affiliated Hospital of Guangxi Medical University,Department
• 出 处: Academic Journal of Xi'an Jiaotong University
• 发表日期: 2008年02期
• 关键词: cathepsin antisense osteosarcoma ASODN invading transfection inhibited targeted

Objective To study the biological effects of cathepsin B phosporothioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection.Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide(ASODN)targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000.The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls.The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot.The invasive capability of MG-63 cells was evaluated by the boydern chamber assay.Results The expression of cathepsin B was obviously inhibited in antisense oligodeoxynucleotide treated cells compared with the control cells.The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups.Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.

Objective To study the biological effects of cathepsin B phosporothioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG- 63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG -63 cells was evaluated by the boydern chamber assay. Results The expression of cathepsin B was obviously inhibited in antisense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusions The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.