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目的:建立测定复方雷锁辛擦剂中间苯二酚、苯酚和水杨酸含量的高效液相色谱法。方法:色谱柱Kromasil C18(4．6 mm×150 mm,5 μm),流动相:甲醇-2％乙酸溶液(40:60),流速1．0 ml·min-1,检测波长:280 nm。结果:间苯二酚在5～25 μg·ml-1,苯酚在10～100μg·ml-1,水杨酸在3～90μg·ml-1范围内与峰面积呈良好的线性关系,相关系数依次为:0．999 9, 0．999 8,0．999 9。回收率依次为:99．5％(RSD0．9％),100．1％(RSD0．6％),99．7％(RSD 1．4％)。结论:本法专属性强,结果准确可靠。适用于复方雷锁辛擦剂的质量控制。
Objective: To establish a HPLC method for the determination of resorcinol, phenol and salicylic acid in Fufang Leihuaxin liniment. METHODS: Kromasil C18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was methanol-2% acetic acid solution (40:60) and the flow rate was 1.0 ml · min-1. The detection wavelength was 280 nm. Results: Resorcinol showed a good linear relationship with the peak area in the range of 5-25 μg · ml-1, phenol at 10-100 μg · ml-1 and salicylic acid in the range of 3-90 μg · ml-1. The correlation coefficient Followed by: 0.999 9, 0.999 8,0.999 9. The recoveries were 99.5% (RSD 0.9%), 100.1% (RSD 0.6%) and 99.7% (RSD 1.4%). Conclusion: This law is specific and accurate and reliable. It is suitable for the quality control of compound Leihuaxin liniment.