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目的:观察膀胱出口梗阻(partial bladder outlet obstruction,PBOO)大鼠膀胱功能及逼尿肌组织氧化应激水平的变化,以及补肾活血方对这些变化的影响。方法:将3月龄雄性Wistar大鼠68只,随机选出12只大鼠作为正常组。剩余大鼠按后述手术方法复制膀胱出口梗阻(BOO)模型,并随机分为4组,每组14只,分别为模型组、补肾活血组、氯化铯组、补肾活血加氯化铯组。于造模成功并分组后第1天起,分别给予相应药物:生理盐水灌胃、补肾活血方灌胃、生理盐水灌胃+皮下注射氯化铯、补肾活血方灌胃+皮下注射氯化铯,正常组同期等体积生理盐水灌胃。给药30 d后,对各组大鼠进行膀胱测压;然后处死各组大鼠,快速取出膀胱并称取湿重;取逼尿肌组织用于检测MDA、SOD、T-AOC等氧化应激各项指标。结果:(1)与正常组相比,模型组(P<0.01)、氯化铯组(P<0.01)膀胱壁组织中MAD水平明显升高,中药组(P<0.01)及中药加氯化铯组(P<0.01)膀胱壁组织中MAD水平则明显低于模型组。(2)模型组(P<0.01)、氯化铯组(P<0.05)及中药加氯化铯组(P<0.01)膀胱壁组织中SOD水平亦明显高于正常组。中药组(P<0.01)膀胱壁组织中SOD水平则明显低于模型组。(3)模型组T-AOC水平明显低于正常组(P<0.05),其它各组与正常组相比,其膀胱壁组织T-AOC水平虽然均有所下降,但均无统计学意义。结论:PBOO可以引起膀胱组织总抗氧化能力下降,并最终导致氧化应激水平上升。补肾活血方可以明显降低大鼠膀胱组织MAD水平,而其对T-AOC的影响似乎并不显著。氯化铯可以部分阻断补肾活血方降低膀胱组织氧化应激水平的作用。
Objective: To observe the changes of bladder function and detrusor tissue oxidative stress in partial bladder outlet obstruction (PBOO) rats and the effects of Bushen Huoxue prescription on these changes. Methods: 68 male Wistar rats of 3 months old were selected and 12 rats were randomly selected as the normal group. The remaining rats were duplicated into bladder outlet obstruction (BOO) according to the operation method described later, and randomly divided into 4 groups (14 rats in each group), model group, Bushen Huoxue group, CsCl group, Bushenhuoxue plus CsCl group . On the first day of successful model and grouping, the corresponding drugs were given respectively: saline, Gushenhuoxue prescription, saline injection + subcutaneous injection of cesium chloride, Bushenhuoxue prescription + subcutaneous injection of cesium chloride , Normal group equivalent volume of normal saline gavage. After administration for 30 days, the rats in each group were subjected to bladder manometry. After that, the rats in each group were sacrificed and the bladder was quickly removed and weighed. The detrusor tissue was taken for detecting the oxidation of MDA, SOD and T-AOC Stimulate the indicators. Results: (1) Compared with the normal group, MAD levels in the model group (P <0.01) and cesium chloride group (P <0.01) Cesium group (P <0.01) MAD in bladder wall tissue was significantly lower than the model group. (2) The SOD level in the bladder wall tissue of the model group (P <0.01), cesium chloride group (P <0.05) and the Chinese medicine plus cesium chloride group (P <0.01) were also significantly higher than those of the normal group. The Chinese medicine group (P <0.01) SOD in the bladder wall tissue was significantly lower than the model group. (3) The level of T-AOC in the model group was significantly lower than that in the normal group (P <0.05). Compared with the normal group, the T-AOC levels in the other groups were not significantly decreased. Conclusion: PBOO can cause the total antioxidant capacity of bladder tissue decreased, and eventually lead to increased levels of oxidative stress. Bushenhuoxue Prescription can significantly reduce the level of MAD in rat bladder tissue, and its effect on T-AOC does not seem to be significant. CsCl can partially block Bushen Huoxue Fang reduce the level of oxidative stress in the bladder tissue.