论文部分内容阅读
目的:从临床及细胞学角度探究miR-449a与胃癌发生的相关性。方法:应用实时荧光定量PCR(qRT-PCR)检测胃癌组织与癌旁周围正常组织中miR-449a表达水平;使用Lipofectamine 2000转染法对MGC803细胞系miR-449a表达进行上调及下调,采用实时细胞动态监测(RTCA)系统及DAPI染色法检测MGC803细胞生长与凋亡情况。结果:(1)相比癌旁周围正常组织,胃癌组织中miR-449a为低表达,其相对表达量为(23.934±4.566,P=0.000);(2)相对于对照组,上调MGC803细胞系miR-449a表达后,RTCA实验结果细胞增殖速度降低(3.947±3.677,P=0.000),DAPI染色结果显示细胞凋亡增加(59.900±8.700,P=0.002);下调miR-449a表达后细胞增殖速度增快(9.005±1.372),凋亡减少(12.500±3.600)。结论:miR-449a具有类似抑癌作用,其低表达与胃癌的发生、发展具有相关性。
Objective: To investigate the relationship between miR-449a and gastric carcinogenesis from the perspective of clinical and cytology. Methods: The expression of miR-449a in gastric cancer tissues and adjacent normal tissues was detected by real-time quantitative PCR (qRT-PCR). The expression of miR-449a in MGC803 cells was up-regulated and down regulated by Lipofectamine 2000. Dynamic monitoring (RTCA) system and DAPI staining were used to detect the growth and apoptosis of MGC803 cells. Results: (1) The relative expression of miR-449a in gastric cancer tissue was (23.934 ± 4.566, P = 0.000) compared with the normal tissue adjacent to cancer. (2) Compared with the control group, MGC803 cell line After miR-449a was expressed, the rate of cell proliferation was decreased by RTCA assay (3.947 ± 3.677, P = 0.000). DAPI staining showed that cell apoptosis increased (59.900 ± 8.700, P = 0.002) (9.005 ± 1.372) and decreased apoptosis (12.500 ± 3.600). Conclusion: miR-449a has a similar anti-tumor effect, and its low expression is associated with the occurrence and development of gastric cancer.