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目的:优选超临界CO2流体萃取技术降低哈蟆油中雌激素含量的工艺条件。方法:采用放射免疫法测定雌激素(雌二醇、雌三醇)含量,以哈蟆油中雌激素的平均去除率为指标,通过正交试验考察萃取温度、萃取时间、萃取压力、夹带剂用量对雌激素CO2超临界萃取工艺的影响。结果:最佳萃取工艺条件为温度50℃,压力30 MPa,乙醇夹带剂用量35 mL,萃取时间4 h;雌激素平均除去率32.12%,1-甲基海因剩余率88.7%。结论:优选的CO2超临界萃取工艺稳定可行,能有效降低哈蟆油中雌激素含量且基本保留了哈蟆油润肺止咳的有效成分。
Objective: To optimize the supercritical CO2 fluid extraction technology to reduce the content of estrogen in Rana oil conditions. Methods: The contents of estrogen (estradiol and estriol) were determined by radioimmunoassay. The average removal rate of estrogen in toad oil was taken as an index. The effects of extraction temperature, extraction time, extraction pressure, entrainer Effect of dosage on the supercritical CO2 extraction of estrogen. Results: The optimal extraction conditions were temperature 50 ℃, pressure 30 MPa, ethanol entrainment 35 mL and extraction time 4 h. The average estrogen removal rate was 32.12% and the residual rate of 1-methylhydantoin was 88.7%. Conclusion: The optimal CO2 supercritical extraction process is stable and feasible, which can effectively reduce the content of estrogen in Rana oil and basically retain the active ingredients of Oviductus Pulcherrima.