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目的探讨脓毒症大鼠肺组织中高迁移率蛋白-1(HMG-1)的表达。方法 50只SD 大鼠随机分为对照组(10只)和试验组(40只),使用逆转录-聚合酶链反应(RT-PCR)法检测肺与空肠组织HMG-1 mRNA的表达,采用Western blot法检测HMG-1蛋白的表达。结果试验组肺、空肠组织中HMG-1 mRNA表达量明显高于对照组(0．866±0．026 vs 0．232±0．009和0．859±0．053 vs 0．227±0．025,P<0．05),试验组肺组织中HMG-1蛋白表达量明显高于对照组(1．060±0．036 vs 0．360±0．006,P<0．05),但各亚组之间差异无统计学意义(P>0．05);空肠组织中HMG-1蛋白表达量与对照组比较,差异无统计学意义(P>0．05)。结论 HMG-1可能是炎症反应的重要介质,在炎症反应的持续发展过程中起一定作用。
Objective To investigate the expression of high mobility group-1 (HMG-1) in the lung tissue of septic rats. Methods Fifty SD rats were randomly divided into control group (n = 10) and experimental group (n = 40). The expression of HMG-1 mRNA in lung and jejunum tissues was detected by reverse transcription polymerase chain reaction The expression of HMG-1 protein was detected by Western blot. Results The expression of HMG-1 mRNA in lung and jejunum in experimental group was significantly higher than that in control group (0.866 ± 0.026 vs 0.232 ± 0.009 and 0.859 ± 0.053 vs 0.227 ± 0. 025, P <0.05). The expression of HMG-1 protein in the lung tissue of the experimental group was significantly higher than that of the control group (1.060 ± 0.036 vs 0.360 ± 0.006, P <0.05) There was no significant difference between each subgroup (P> 0.05). The expression of HMG-1 protein in jejunum was not significantly different from that in control group (P> 0.05). Conclusion HMG-1 may be an important mediator of inflammation and play a role in the sustained development of inflammatory response.