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矿泉水水井因污染而在取水管道内壁形成大量的褐色粘液状粘泥 ,显微形态观察表明 ,典型样品中的粘泥主要为细菌污染大量繁殖形成的生物膜。样品经HCl处理后 ,其中黄褐色晶体、霉菌样丝状体消失 ,显示晶体和丝状体为金属沉积物 ;样品滴加亚铁氰化钾和HCl,出现普鲁士蓝沉淀 ,证明了样品中的金属沉积物是含铁物质 ,从而推断取水管内壁附着物主要为铁细菌大量繁殖 ,氧化铁、沉积铁而形成的生物膜。从生物膜中取样进行微生物分离培养 ,铁细菌检出阳性 ,固体培养物同样与亚铁氰化钾反应出现普鲁士蓝沉淀 ,且显微形态与样品中微生物一致 ,进一步证明水管内壁附着物中污染微生物主要为铁细菌。
Mineral water wells due to pollution in the water intake pipe wall to form a large number of brown mucus-like sticky mud, microscopic morphological observations showed that the typical samples of sludge mainly for bacterial contamination of the proliferation of the formation of biofilms. The samples were treated with HCl, in which the brown crystals and mold-like filaments disappeared, indicating that the crystals and filaments were metal deposits. The sample was precipitated with potassium ferrocyanide and HCl, and Prussian blue precipitation was observed. The metal deposit is an iron-containing material, and thus it is inferred that the attachment of the internal wall of the water-drawing pipe is mainly a biofilm formed by the large population of iron bacteria, iron oxide and iron deposition. Samples were taken from the biofilm for microbial isolation and culture, positive for iron bacteria, and solid culture was also observed to react with potassium ferrocyanide to produce Prussian blue precipitation, and the microscopic morphology was consistent with that of the samples, further confirming contamination of the pipe wall attachments Microbes are mainly iron bacteria.