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目的:建立适合于心血管疾病研究的复合因素致大鼠动脉粥样硬化模型。方法:健康SD雄性大鼠,分为10组,空白对照组、高脂对照组、L-met(低)组和L-met(高)组、高脂+L-met(低)组(4、6、8周)、高脂+L-met(高)组(4、6、8周)。检测血清TC、TG、HDL,检测血浆同型半胱氨酸(Hcy)、单核细胞趋化因子1(MCP-1)和白介素6(IL-6),分离各组大鼠主动脉弓,置于10%中性福尔马林中固定,作HE染色。结果:试验后,高脂对照组和高脂+L-met(低、高)(4、6、8周)组大鼠血清TC水平均明显增高;高脂对照组和高脂+L-met(低、高)(4周)组大鼠血清TG水平均明显增高;高脂对照组、L-met(低、高)和高脂+L-met(低、高)(4、6、8周)组大鼠血浆Hcy水平均明显增高;L-met(低、高)、高脂+L-met(低)(6、8周)组和高脂+L-met(高)(4、6、8周)大鼠血浆MCP-1水平均明显增高,同空白对照组相比P<0.05。L-met(低)组和高脂+L-met(低)组(4周)大鼠可见早期动脉粥样硬化改变,L-met(高)组、高脂+L-met(低)组(6、8周)和高脂+L-met(高)组(4、6周)大鼠可见中、晚期AS病理改变,高脂+L-met(高)组(8周)大鼠则出现晚期动脉粥样硬化改变。结论:通过L-met灌胃,联合应用高脂饲料喂养的方法可建立较理想的动脉粥样硬化模型。
OBJECTIVE: To establish a model of atherosclerosis induced by multiple factors in cardiovascular diseases. Methods: Healthy male Sprague Dawley rats were randomly divided into 10 groups: control group, high-fat control group, L-met group and L-met group , 6,8 weeks), high fat + L-met (high) group (4,6,8 weeks). Serum levels of TC, TG and HDL were measured and plasma homocysteine (Hcy), monocyte chemotactic factor 1 (MCP-1) and interleukin 6 (IL-6) were detected. % Neutral formalin fixation, HE staining. Results: After the experiment, the serum TC levels in the high fat control group and the high fat + L-met (low, high) (4, 6 and 8 weeks) groups were significantly increased; high fat control group and high fat + L-met (Low, high) (4 weeks) group were significantly higher than those in the control group (P <0.05). The levels of plasma Hcy in L-met (low, high), high fat + L-met 6, 8 weeks) plasma MCP-1 levels were significantly increased, compared with the blank control group P <0.05. Early atherosclerosis was observed in the L-met (low) and high-fat + L-met (low) groups (4 weeks) (6, 8 weeks) and hyperlipidemia + L-met (4 weeks, 6 weeks), the pathological changes of middle and late stage AS were observed. The rats in high fat + L-met group Advanced atherosclerosis appears. Conclusion: The optimal model of atherosclerosis can be established by L-met gavage and fed with high fat diet.