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以甘蓝型黄籽油菜GH06和甘蓝型黑籽油菜中油821为亲本杂交,后代通过“一粒传法”连续自交7代构建重组自交系,2007年分别在重庆市北碚区和万州区2个试验基地种植重组自交系群体,利用本实验室已构建的遗传连锁图谱和复合区间作图法(CIM),对种皮木质素含量进行测定及QTL定位分析。结果表明,在2个环境中共检测到12个种皮木质素含量相关的QTL,分别位于4个不同的连锁群,单个QTL可解释性状表型变异的4.50%～8.79%;在第3连锁群上检测到1个QTL与同一标记EM19ME23/130连锁,其余10个QTL位置不同;在北碚检测到的QTL主要分布于第20连锁群,在万州检测到的QTL主要位于第3连锁群;部分种皮木质素的QTL与种胚类黄酮和种皮色泽的QTL位于相近区间,在北碚和万州种皮木质素含量与种胚类黄酮存在极显著和显著正相关关系。甘蓝型油菜种皮木质素含量表现为多基因控制的数量性状,基因表达受环境影响较大;油菜种皮木质素合成和类黄酮的积累可能受相同关键基因调控或者具有部分相同的合成代谢途径。
The recombinant inbred lines were constructed with the yellow-seeded GH06 and the Brassica napus Zhongyou 821, and the offsprings were self-inbred for 7 generations through the “one grain transfer method”. In 2007, the recombinant inbred lines were constructed in Beibei District of Chongqing and Wanzhou The population of recombinant inbred lines was planted in two experimental sites in the district. The lignin content of seed coat and QTL mapping were analyzed by genetic linkage map and composite interval mapping (CIM) constructed in our laboratory. The results showed that 12 QTLs related to seed lignin content were detected in two environments, which were located in four different linkage groups. A single QTL explained 4.50% ~ 8.79% of the phenotypic variation in traits. In the third linkage group QTLs detected in Beibei were mainly distributed in the 20th linkage group, QTLs detected in Wanzhou were mainly located in the third linkage group; some QTLs detected in Wanzhou were mainly located in the third linkage group; The QTLs for lignin and the QTLs for embryo flavonoids and seed coat color were located in similar intervals. The lignin content in the seed coat of Beibei and Wanzhou had a significant and significant positive correlation with the QFs. The lignin content of seed coat in B. napus showed quantitative traits controlled by multiple genes, and the gene expression was greatly affected by the environment. The accumulation of lignin and flavonoids in seed coat of rapeseed may be controlled by the same key genes or with some of the same metabolic pathways .