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试验研究了腐殖酸钠对切花菊试管苗生长发育的影响。结果表明,1)腐殖酸钠能够促进愈伤组织的生长,较适合的培养基为MS+6-BA 3毫克/升+NAA 1毫克/升 +0.76%腐殖酸钠100毫升/升。2)腐殖酸钠能够较显著地提高芽苗分化能力,在采用MS+6-BA 3毫克/升+NAA 0.1毫克/升+0.76%腐殖酸钠100毫升/升培养基的情况下,芽苗增殖系数为28.3。3)适当降低激素水平,增加腐殖酸钠,结果明显地促进了芽苗生长,培养一月后的茅苗株高14.1厘米,茎粗0.2厘米。较好的培养基为MS+6-BA0.5毫克/升+NAA 0.1毫克/升+0.76%腐殖酸钠150毫升/升。4)在1/2MS培养基上使用腐殖酸钠,芽苗发根数可以增加一倍。较适合的培养基为1/2MS+IBA 0.5毫克/升+0.76%腐殖酸钠50毫升/升(糖减半)。 本试验为腐殖酸类物质在组织培养中的应用提供了有益的启示。
The effects of sodium humate on the growth and development of cut chrysanthemum plantlets were studied. The results showed that 1) sodium humate could promote the growth of callus, more suitable medium was MS + 6-BA 3 mg / L + NAA 1 mg / L + 0.76% sodium humate 100 mL / L. 2) Sodium humate can significantly improve shoot differentiation ability. In the case of MS + 6-BA 3 mg / L + NAA 0.1 mg / L + 0.76% sodium humate 100 mL / L medium, Sprouts multiplication coefficient of 28.3.3) appropriate to reduce the hormone levels, increased sodium humate, the results significantly promoted the growth of shoots, the culture of January after the Miao Miao height of 14.1 cm, stem diameter 0.2 cm. The preferred medium is MS + 6-BA 0.5 mg / L + NAA 0.1 mg / L + 0.76% Sodium humate 150 mL / L. 4) Using sodium humate in 1 / 2MS medium, the number of shoots can be doubled. A more suitable medium is 1 / 2MS + IBA 0.5 mg / L + 0.76% Sodium Humate 50 mL / L (sugar halved). This experiment provides a useful enlightenment for the application of humic substances in tissue culture.