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目的探讨利福平对脓肿分枝杆菌L型的诱导作用。方法将脓肿分枝杆菌分别接种于含256μg/mL利福平和无利福平的结核分枝杆菌快速液体培养基中培养。含利福平的培养物经0.45μm孔径滤膜过滤,滤液接种于无利福平的结核分枝杆菌快速液体培养基内返祖培养。将含利福平和无利福平的培养物及返祖菌进行细胞壁染色和透射电镜观察其细胞壁完整性,扫描电镜观察其表面微观结构。含利福平的培养物转种L型菌琼脂平板培养,无利福平的培养物和返祖菌转种营养琼脂平板培养,观察其菌落形态。对诱导前接种菌和返祖菌的16S rDNA进行鉴定。结果在含256μg/mL利福平浓度的结核分枝杆菌快速液体培养基中培养的脓肿分枝杆菌的细胞壁缺失,形态为球形,L型细菌琼脂平板上菌落呈典型油煎蛋样,而无利福平的结核分枝杆菌快速液体培养基中培养的脓肿分枝杆菌细胞壁完整,形态为杆状,营养琼脂平板上呈圆形菌落。256μg/mL利福平浓度的结核分枝杆菌快速液体培养基中培养的脓肿分枝杆菌经返祖后,返祖菌细胞壁完整,形态为杆状,营养琼脂平板上菌落也呈圆形。16S rDNA鉴定返祖菌与诱导前接种菌的同源性达100%,为同一种细菌,即脓肿分枝杆菌。结论利福平成功诱导脓肿分枝杆菌L型。
Objective To investigate the induction of rifampicin on L type of Mycobacterium abscess. Methods Mycobacterium abscess was inoculated into Mycobacterium tuberculosis rapid liquid medium containing 256 μg / mL rifampicin and no rifampicin respectively. The culture containing rifampicin was filtered through a 0.45 μm pore filter, and the filtrate was inoculated into the mycobacterium tuberculosis rapid liquid medium without rifampicin for the ancestral culture. The cultures containing Rifampicin and Rifampicin, and the ancestor of U. virens were stained with cell wall and examined by transmission electron microscopy for cell wall integrity. The surface microstructure was observed by scanning electron microscopy. Rifampicin-containing cultures were transformed into L-type agar plates without rifampicin and reconstituted nutrient agar plates. The colony morphology was observed. The 16S rDNA of the inoculated and the returned progenies were identified. Results Mycobacterium abscess was cultured on Mycobacterium tuberculosis rapid liquid medium containing 256 μg / mL of rifampicin at a loss of cell wall with a spherical shape. The colonies on the L-type agar plates showed typical omelettes without Rifampicin Mycobacterium tuberculosis rapid liquid medium cultured Mycobacterium abscess cell wall integrity, rod-shaped nutrients, nutrient agar plate was round colonies. Mycobacterium tuberculosis was cultured in Mycobacterium tuberculosis fast liquid medium with a concentration of 256μg / mL rifampicin. After returning to the ancestor, the cell wall of the anaerobic bacterium was intact in the shape of a rod, and the colonies on the nutrient agar plate were also round. 16S rDNA identification back to the original strain of bacteria and inoculum of 100% identity, the same bacteria, namely Mycobacterium abscess. Conclusion Rifampicin successfully induced Mycobacterium abscess L type.