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白血病是我国儿童恶性肿瘤中最多见的类型，尽管新的治疗手段有所应用，但化疗仍然是白血病的主要治疗方法，而影响化疗药物疗效的重要因之一是多源耐药（multidrug re－sistance）基因的高水平表达，其结果可以产生对多种结构、作用机制完全不同的药物同时耐药，因此对联合应用的化疗方案造成不良影响。多源耐药基因编码一个分子量为 170 kb的细胞膜糖蛋白（P一糖蛋白），在正常细胞中它是一个ATP依赖性的胞内脂质物质输出泵，起到转运胞内代谢产物的作用。抗肿瘤药物中：烷化剂类、植物碱类、抗肿瘤抗生素及激素类都是脂质（或亲脂疏水）性物质，多源耐药基因的蛋白质产物就是通过将这些药物泵出细胞而降低了药物在细胞内的浓度而使肿瘤细胞产生耐药性。我们应用逆转录PCR结合同位素定量分析，对 32例儿童白血病患者的多源耐药基因表达水平进行了研究。复发病人 MDR的表达里高水平（0． 474土 0． 087，P＜0． of），而初发病人未接受化疗者，MDR的表达则呈低水平（0． 157士0． 148），PMO． 05），缓解期病人的 MDR表达水平介于两者之间，该研究为探讨多源耐药基因表达水平与临床化疗之间的相关性及逆转克服多源耐药性药物的应用，提供了一定的理论依据。
Leukemia is the most common type of childhood malignant tumor in our country. Although new therapeutic methods are used, chemotherapy is still the main treatment for leukemia. One of the important factors affecting the curative effect of chemotherapy drugs is multidrug re- sistance gene expression, the results can produce a variety of structures, mechanisms of action of drugs at the same time completely different, and thus adversely affect the combined application of chemotherapy. Multi-drug resistance gene encodes a 170kb molecular weight cell membrane glycoprotein (P-glycoprotein), which is an ATP-dependent intracellular lipid export pump in normal cells that plays a role in transporting intracellular metabolites . Antitumor drugs: alkylating agents, plant alkaloids, antitumor antibiotics and hormones are lipid (or lipophilic) hydrophobic substances, protein products of multi-drug resistance genes by pumping these drugs out of cells and Reduce the drug concentration in the cell and make the tumor cells produce drug resistance. We used reverse transcription PCR combined with quantitative analysis of isotopes, 32 cases of childhood leukemia multi-drug resistance gene expression levels were studied. The recurrence of patients with high expression of MDR (0. 474 ± 0. 087, P <0. Of), but no initial chemotherapy in patients with chemotherapy, MDR expression was low (0. 157 ± 0. 148) PMO. 05), MDR expression in patients with remission was in between the two, the study to explore multi-drug resistance gene expression levels and the clinical relevance of chemotherapy and reversal of multi-drug resistance to overcome the use of the provision of A certain theoretical basis.