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目的:探讨喉鳞状细胞癌(LSCC)组织中EGFL7的表达与喉癌侵袭转移的关系及其可能机制。方法:提取33例新鲜喉癌组织和33例相应的癌旁组织(PNTT)的总RNA,用RT-PCR方法测定EGFL7基因表达,Western-blot方法测定EGFL7蛋白的表达情况;用CD31抗体进行免疫组织化学检测喉癌组织中微血管密度(MVD),并分析EGFL7的表达及MVD与临床病理特征的关系。结果:33例新鲜喉癌标本中EGFL7mRNA和EGFL7蛋白表达均显著高于癌旁组织(P<0.01)。EGFL7mRNA和EGFL7蛋白表达具有显著相关性(r=0.786,P<0.01)。喉癌组织中EGFL7mRNA、EGFL7蛋白的表达水平和MVD与喉癌组织的临床分期、肿瘤的直径大小及有无淋巴结转移等密切相关(P<0.05),而与患者的性别、年龄、肿瘤原发部位及分化程度等无关(P>0.05)。结论:EGFL7可能通过影响血管生成参与了喉癌的发展。EGFL7蛋白可能成为预测喉癌的病情发展及指导临床治疗的标志物之一。
Objective: To investigate the relationship between the expression of EGFL7 and the invasion and metastasis of laryngeal carcinoma in laryngeal squamous cell carcinoma (LSCC) and its possible mechanism. Methods: The total RNA of 33 cases of fresh laryngeal carcinoma and 33 cases of corresponding para-cancerous tissues (PNTT) was extracted. The expression of EGFL7 gene was detected by RT-PCR and the expression of EGFL7 protein by Western-blot. The microvessel density (MVD) in laryngeal carcinoma tissues was detected by histochemistry. The expression of EGFL7 and the relationship between MVD and clinicopathological features were analyzed. Results: The expression of EGFL7mRNA and EGFL7 protein in 33 cases of fresh laryngeal carcinoma were significantly higher than those in paracancerous tissues (P <0.01). There was a significant correlation between EGFL7 mRNA and EGFL7 protein expression (r = 0.786, P <0.01). The expression levels of EGFL7mRNA and EGFL7 in laryngeal carcinoma and MVD were closely related to the clinical stage of laryngeal carcinoma, the diameter of the tumor and the lymph node metastasis (P <0.05), but not with the gender, age, Location and degree of differentiation has nothing to do (P> 0.05). Conclusion: EGFL7 may be involved in the development of laryngeal carcinoma by affecting angiogenesis. EGFL7 protein may be one of the markers to predict the progression of laryngeal cancer and guide the clinical treatment.