论文部分内容阅读
目的:研究柴胡注射液在细胞培养中抑制呼吸道合胞病毒(RSV)的作用.方法:用细胞培养法,采取Vero细胞感染病毒2小时后给药,在显微镜下观察细胞病变情况.结果:柴胡注射液组与病毒对照组相比,病变程度有显著差异,柴胡注射液抑制RSV的最大无毒浓度(TDo),半数有效浓度(IC_(50)),最小有效浓度(MTC)和治疗指数(TI)分别为1000μg/ml,500μg/ml,250μg/ml和4.结论:柴胡注射液具有明显抑制RSV的作用.
OBJECTIVE: To study the effect of Bupleurum chinense injection on the inhibition of respiratory syncytial virus (RSV) in cell culture. Methods: Using cell culture method, Vero cells were infected with virus for 2 hours, and the cell lesions were observed under microscope. There was a significant difference in the extent of lesions between the Bupleurum injection group and the virus control group. Bupleurum injection inhibited RSV’s maximum non-toxic concentration (TDo), half effective concentration (IC 50), and minimum effective concentration (MTC). Therapeutic index (TI) was 1000μg/ml, 500μg/ml, 250μg/ml and 4. Conclusion: Bupleurum injection can inhibit RSV obviously.