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The binding mode of Eu(phen) 3+3 with DNA is studied by fluorescence and voltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it is concluded that the mode of interaction between Eu(phen)3+3 and DNA is intercalation bind by inserting the phen ?-? conjugate surface between the base pairs of the DNA duplex. Voltammetry was used to confirm the results obtained from the fluorescence method, the result from both methods agrees with each other. The binding constant (K) and the binding site size (ns) were calculated from voltammetric data such as the shifts in potential and limiting currents in the process of adding DNA, according to the positive shifts of peak potential. It was considered that the complex of the +2 ion interacted more favorably with the nucleotide bases than that of the +3 ion by hydrophobic interaction.
The binding mode of Eu (phen) 3 + 3 with DNA is studied by fluorescence and voltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it is concluded that the mode of interaction between Eu (phen) 3 + 3 and DNA is intercalation bind by inserting the phen? -? conjugate surface between the base pairs of the DNA duplex. Voltammetry was used to confirm the results obtained from the fluorescence method, the result from both methods agrees with each other. The binding constant (K) and the binding site size (ns) were calculated from voltammetric data such as the shifts in potential and limiting currents in the process of adding DNA, according to the positive shifts of peak potential. It was considered that the complex of the +2 ion interacted more favorably with the nucleotide bases than that of the +3 ion by hydrophobic interaction.