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[目的]研究5-氟尿嘧啶(5-FU)与曲古抑菌素A(TSA)单独及联合应用对人肝癌细胞HepG2生长的抑制作用,并初步探讨其与NF-κBp65和AKT表达的关系。[方法]以MTT法观察5-FU或(和)TSA对HepG2细胞生长的影响,Hoechst染色法分析细胞凋亡情况,Westernblot检测NF-κBp65和AKT蛋白表达水平的变化。[结果]与对照组相比,5-FU与TSA均可导致处理的细胞增殖速度减慢,且抑制效应随药物剂量的增加而增强,两药联合应用可明显增强其抑制作用,促进HepG2细胞凋亡,并降低NF-κBp65和AKT蛋白的表达(P﹤0.01)。[结论]TSA和5-FU联合应用可能通过协同调控下调NF-κBp65与AKT蛋白表达而抑制HepG2细胞的增殖。
[Objective] To investigate the inhibitory effect of 5-fluorouracil (5-FU) and trichostatin A (TSA) alone and in combination on the growth of HepG2 human hepatocellular carcinoma cell line HepG2 and its relationship with the expression of NF-κBp65 and AKT. [Method] The effect of 5-FU or TSA on the growth of HepG2 cells was observed by MTT assay. The apoptosis of HepG2 cells was analyzed by Hoechst staining. The expressions of NF-κBp65 and AKT protein were detected by Western blot. [Results] Compared with the control group, both 5-FU and TSA could slow down the proliferation of the treated cells, and the inhibitory effect increased with the increase of the drug dosage. The combination of the two drugs could obviously enhance the inhibition and promote the proliferation of HepG2 cells Apoptosis, and decreased the expression of NF-κBp65 and AKT protein (P <0.01). [Conclusion] The combination of TSA and 5-FU may inhibit the proliferation of HepG2 cells through the down-regulation of NF-κBp65 and AKT protein expression.