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一氧化氮(NO)在细胞信号传递中起着重要作用,参与调节心血管、神经和免疫系统功能,与许多疾病的发病过程有关。一氧化氮合酶(nitric oxidesynthase,NOS)是NO合成过程的关键酶。目前已知有3种异构体:神经型(neuronal,nNOS),内皮型(endothelial,eNOS),及诱生型(inducible,iNOS)。前两者又称为原生型(constitutive,cNOS),其活性依赖细胞内钙离子浓度的增加,cNOS活化后可生成少量NO,引起局部效应:而iN-OS活性不依赖钙离子浓度,受多种细胞因子(TNF-α、IFN-γ、IL-1β))和 LPS等诱导激活,可产生大量NO,导致广泛效应,iNOS存在于多种细胞的胞浆内,故测定iNOS具有重要意义。
Nitric oxide (NO) plays an important role in cell signaling and is involved in the regulation of cardiovascular, neurological and immune system functions and is involved in the pathogenesis of many diseases. Nitric oxide synthase (NOS) is a key enzyme in NO synthesis. Three isoforms are currently known: neuronal (nNOS), endothelial (eNOS), and inducible (iNOS). The former two, also known as constitutive (cNOS), their activity depends on the increase of intracellular calcium concentration. After activation of cNOS, a small amount of NO can be generated, causing local effect: while iN-OS activity is independent of calcium concentration, Induced activation by cytokines (TNF-α, IFN-γ, IL-1β)) and LPS can produce a large number of NO, leading to a wide range of effects, iNOS exists in the cytoplasm of a variety of cells, so the determination of iNOS is of great significance.