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目的 :探讨铅对小鼠生殖作用的影响.方法 :采用小鼠卵母细胞体外培养、体外受精的方法研究了铅对小鼠卵母细胞的成熟和体外受精的影响.结果 :铅对小鼠卵母细胞生发泡破裂没有影响 ,但可以抑制卵母细胞第一极体的释放 ,影响卵母细胞的存活率并可降低体外受精率和超排卵的卵母细胞数量.随着培养时间的延续 ,卵母细胞的第一极体的释放率和体外受精率都有显著提高.结论 :铅可以破坏卵母细胞减数分裂进行 ,降低卵母细胞的受精能力 ,影响小鼠的正常生殖功能“,”Objective:To study the effect of lead on the mouse fertility.Methods:The meiotic maturation and IVF of mouse oocytes were studied with Pb(NO 3) 2 using in vitro culture and in vitro fertilization(IVF) of mouse oocyte. Results:The results showed that lead could significantly reduce the number of superovulation oocytes, the rates of IVF and the quality and viability of mouse oocyte, inhibit the meiotic maturation and the extruding of the first polar body of mouse oocytes, though there were little impact on the GVBD. The results also showed that the extruding of the first polar body and IVF rates may be increased obviously by the culture of oocytes and the rate of IVF in normal culture medium.Conclusion:The results indicated that lead may be hazardous to the process of meiotic maturation and may affect the reproduction of mouse, reduce the fertility and reproduction ability.