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目的 探讨玻璃体腔注射碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对实验性视网膜缺血再灌注损伤的治疗作用。方法 采用升高眼内压的方法 ,制作实验性视网膜缺血再灌注损伤大鼠模型。将Wistar大鼠随机分为正常组、缺血组及治疗组。再灌注开始时 ,缺血组大鼠玻璃体腔内注入平衡盐溶液 ,治疗组注入bFGF 2 μg。观察再灌注后不同时间段各组鼠视网膜组织学及超微结构变化 ,光镜下计数视网膜神经节细胞 (retinalganglioncells,RGCs) ,应用图像分析系统测量视网膜内层厚度。结果 视网膜缺血再灌注早期 ,治疗组大鼠视网膜水肿较缺血组轻 ,各时间段治疗组大鼠视网膜内层厚度均较缺血组厚 ,治疗组大鼠RGCs数目多于缺血组。再灌注后 16 8h ,缺血组大鼠神经纤维层厚度及RGCs数目明显低于正常组 ,而治疗组大鼠神经纤维层厚度及RGCs数目与正常组比较 ,差异无显著意义 (P <0 0 5 )。再灌注后 2 4h ,缺血组大鼠RGCs核膜肿胀 ,线粒体嵴模糊不清 ,可见凋亡小体 ,神经纤维中微管模糊、减少甚至消失 ;而治疗组仅部分核膜轻度肿胀 ,胞浆内细胞器丰富 ,线粒体及微管结构较清楚。结论 大鼠玻璃体腔注射bFGF对实验性视网膜缺血再灌注损伤具有治疗作用。
Objective To investigate the therapeutic effect of basic fibroblast growth factor (bFGF) injected into the vitreous cavity on experimental retinal ischemia-reperfusion injury. Methods The method of raising intraocular pressure was used to make experimental rat model of retinal ischemia-reperfusion injury. Wistar rats were randomly divided into normal group, ischemia group and treatment group. At the beginning of reperfusion, balanced saline solution was injected into the vitreous cavity of ischemic rats. The treatment group was given 2 μg of bFGF. Retinal histology and ultrastructure were observed at different time points after reperfusion, and retinal ganglion cells (RGCs) were counted under light microscope. The thickness of retinal inner layer was measured by image analysis system. Results In the early stage of retinal ischemia-reperfusion, retinal edema in the treatment group was lighter than that in the ischemic group. The retina thickness in the treatment group was higher than that in the ischemia group. The number of RGCs in the treatment group was more than that in the ischemia group. At 16 h after reperfusion, the number of nerve fiber layer and the number of RGCs in the ischemic group were significantly lower than those in the normal group. There was no significant difference in the thickness of the nerve fiber layer and the number of RGCs in the treatment group compared with the normal group (P <0 0 5). At 24 h after reperfusion, the nuclear membrane swelling and mitochondrial cristae were unclear in RGCs of ischemic rats, apoptotic bodies and microtubules in neurofibrils were vague and diminished or even disappeared. While in the treatment group, only some of the nuclear membranes swelled slightly, Cytoplasmic organelles rich mitochondria and microtubule structure more clearly. Conclusion The injection of bFGF into the vitreous of rats has a therapeutic effect on experimental retinal ischemia-reperfusion injury.