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为了探讨无融合水稻筛选指标,用透明一染色法观察了数以千计的水稻子房。水稻的多胚有几种表型。一种情况是一个胚是从卵细胞通过受精而产生的,而另一个胚可能是从助细胞产生的,这种情况下往往形成双苗。由于2个胚之间竞争的缘故,有时一个胚较另一个胚大,在这种情况下,一苗长,一苗短的情况就能观察到。水稻的不定胚也曾经观察到,但是频率非常低。正在发育的不定胚正在形成根、叶原基,位于胚囊的侧部,而不在珠孔端,在这种情况下,合子胚可能已经败育。因而,相应地可以观察到从种子中部侧生的幼苗,有时,合子胚仍能发育,这样一个苗从珠孔端长出,而另一个从种子侧部长出的双苗就能观察到,这种从种子背部中间长出幼苗的形态学特点,有可能作为无融合水稻筛选的形态指标。进而,如果能根据体细胞胚和合子胚休眠的不同特点,还可能从时间上进一步去区分它们。
In order to investigate the index of non-fusion rice screening, thousands of rice ovaries were observed by the method of transparent-one staining. There are several phenotypes in polygenes in rice. In one case, one embryo is produced from the egg by fertilization, while the other embryo may be derived from the helper, which in this case often forms double shoots. Due to the competition between the two embryos, sometimes one embryo is larger than the other embryo, in this case, a seedling length, a seedling short situation can be observed. Adventitious embryos in rice have also been observed, but at very low frequencies. The developing adventitious embryos are forming roots and leaf primordia, located on the side of the embryo sac, not at the ends of the peritoneal cavity, in which case zygotic embryos may have been aborted. Accordingly, seedlings emerging laterally from the middle of the seed can correspondingly be observed, and in some cases zygotic embryos can still develop so that one seedling emerges from the end of the micropyle and the other seedling grown from the side of the seed can be observed The morphological characteristics of seedlings grown from the middle of the back of the seed may serve as morphological indicators for screening of non-fusion rice. Furthermore, it is also possible to further distinguish them from each other in terms of the different characteristics of somatic and zygotic embryos’ dormancy.