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目的检测海口市售牡蛎感染诺如病毒的情况,为诺如病毒防治提供参考。方法 2015年9月-2016年5月,在海口市随机抽样,采集牡蛎标本,提取病毒核酸,应用实时荧光定量PCR检测诺如病毒核酸,进行诺如病毒GⅠ群或GⅡ群基因分型。以脊髓灰质炎疫苗中的病毒作为标准品检测病毒的回收率。结果牡蛎标本220份,分别来自广东湛江、广西北海、海南海口和儋州。经实时荧光定量PCR检测6份标本诺如病毒核酸阳性,阳性率为2.73%;其中4份标本同时携带诺如病毒GⅠ群和GⅡ群,2份仅GⅡ群阳性。冬季和春季采集的标本阳性率分别为5.88%(4/68)和3.64%(2/55)。广东湛江和海南海口的牡蛎诺如病毒阳性率分别为9.80%(5/51)和1.28%(1/78),其他两地的牡蛎未检出诺如病毒。以脊髓灰质炎疫苗作为标准品验证诺如病毒的平均回收率为11.46%。结论海口市市售牡蛎受到诺如病毒污染,有关部门应采取相应措施,加强市售海产品管理,降低人群感染诺和病毒的风险。
Objective To detect the presence of Norovirus infected with commercially available oysters in Haikou and provide a reference for prevention and treatment of Norovirus. Methods From September 2015 to May 2016, samples of oyster were collected randomly from Haikou City for extraction of viral nucleic acids. Real-time PCR was used to detect Norovirus nucleic acids to genotype GⅠ or GⅡ of Norovirus. The virus in the polio vaccine was used as a standard to detect the virus recovery rate. Results Oyster samples 220, respectively, from Zhanjiang in Guangdong, Guangxi Beihai, Hainan Haikou and Danzhou. Real-time fluorescence quantitative PCR detection of 6 samples Norovirus nucleic acid positive, the positive rate was 2.73%; 4 specimens carrying norovirus GⅠ and G Ⅱ group, 2 only G Ⅱ group positive. The positive rates of specimens collected in winter and spring were 5.88% (4/68) and 3.64% (2/55) respectively. The positive rates of Norovirus in Oyster from Zhanjiang, Guangdong Province and Haikou in Hainan Province were 9.80% (5/51) and 1.28% (1/78), respectively. Norovirus was not found in the other two oysters. The average recovery of Norovirus was 11.46% with polio vaccine as a standard. Conclusions The oysters on the market in Haikou are contaminated with norovirus, and the relevant departments should take corresponding measures to strengthen the management of marketed seafood and reduce the risk of Nosocomial infection with norovirus.