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目的:探讨喉癌患者中缺氧诱导因子-1α(HIF-1α)、环氧合酶-2(COX-2)与血管内皮生长因子(VEGF)表达水平与喉癌血管生成的关系及临床意义。方法:通过免疫组织化学SP法对64例喉癌标本中HIF-1α、COX-2与VEGF及微血管密度(MVD)进行了检测。结果:喉癌中HIF-1α、COX-2蛋白及VEGF的阳性表达率分别为65.63%(42/64)、68.75%(44/64)和71.88(46/64),MVD为35.79±9.49。喉癌HIF-1α、COX-2和VEGF与MVD表达均呈正相关(P<0.05)。喉癌中HIF-1α和COX-2与VEGF之间存在正相关性(P<0.05)。在有淋巴结转移的肿瘤中HIF-1α、COX-2和VEGF阳性表达及MVD明显高于非转移组(P<0.05)。结论:HIF-1α、COX-2和VEGF参与喉癌的血管生成,HIF-1α通过调节下游基因的表达(COX-2及VEGF),在喉癌血管生成中发挥重要作用,共同参与喉癌颈淋巴结转移过程。
Objective: To investigate the relationship between the expression of hypoxia-inducible factor-1α (HIF-1α), cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) and angiogenesis in laryngeal carcinoma in patients with laryngeal cancer and its clinical significance . Methods: The expressions of HIF-1α, COX-2, VEGF and microvessel density (MVD) in 64 specimens of laryngeal cancer were detected by immunohistochemical SP method. Results: The positive expression rates of HIF-1α, COX-2 protein and VEGF in laryngeal carcinoma were 65.63% (42/64), 68.75% (44/64) and 71.88 (46/64), respectively. The MVD was 35.79 ± 9.49. The expressions of HIF-1α, COX-2, VEGF and MVD in laryngeal carcinoma were positively correlated (P <0.05). There was a positive correlation between HIF-1α, COX-2 and VEGF in laryngeal carcinoma (P <0.05). The positive expression of HIF-1α, COX-2 and VEGF and the MVD in tumor with lymph node metastasis were significantly higher than those in non-metastasis group (P <0.05). Conclusions: HIF-1α, COX-2 and VEGF are involved in the angiogenesis of laryngeal carcinoma. HIF-1α plays an important role in the angiogenesis of laryngeal carcinoma by regulating the expression of downstream genes (COX-2 and VEGF) Lymph node metastasis process.