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Objective:To observe the difference in fatty degree,glucose-lipid disorder and adipose-hormones between diet induced obesity(DIO) rats and diet induced obesity resistance(DIO-R) rats,and to explore the effect and acting mechanism of Chinese drugs for strengthening Pi(脾,CD-SP) and those for both strengthening Pi and dissolving phlegm(CD-SPDP) in inhibiting obesity.Methods:Excepting eight rats allocated in the blank control group,the other 54 rats were fed with high-lipid forage for 12 weeks to establish models of obesity. Finally,30 DIO rats and 8 DIO-R rats(shown by their body weight) were obtained.The DIO rats were divided into three groups,which were given gastric perfusion,respectively,with normal saline(Group A),CD-SP(Group B),and CD-SPDP(Group C).Fourteen weeks later,the animals’ body weight(BW),length(BL),blood levels of fasting insulin(Fln),fasting glucose(FBG),triglyceride(TG),cholesterol(TC),leptin(LP),neuropeptide Y(NPY), C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),adiponectin(AN),and resistin(RS) were measured; insulin resistance index(IRI) was calculated,and the degree of obesity and lipid content in abdominal cavity of rats were estimated.Moreover,the levels of LP,CRP,TNF-α,AN and RS in homogenate of rats’ adipose tissues(ATH) were determined.Results:After 12 weeks of high-lipid diet,the BW of DIO rats was higher than that of normal or DIO-R rats.After a 14-week continuous high-lipid diet feeding,in DIO rats,BW,lipid coefficient (LC),and IRI were significantly increased(P<0.01);serum levels of TNF-α,LP and AN were lower,NPY was higher,while the ATH levels of LP and AN were lower and TNF-αwas higher in DIO rats than in DIO-R rats (P<0.05 or P<0.01);blood levels of FBG and lipids in DIO rats showed an increasing trend but was statistically insignificant(P>0.05);no significant difference was found in serum levels of CRP and RS due to the overly high data dispersion.Comparisons of the 3 DIO groups showed that BW,body weight index(BWI),LC and IRI were significantly lowered after treatment(P<0.01) in Group C,while these indexes were not significantly different between Group A and B;the serum levels of TNF-α,LP,and AN increased,NPY decreased in Group B and C, ATH levels of LP and AN increased,and TNF-αdecreased in the two groups;but NPY,LP,and AN in blood and ATH were higher in Group C than those in Group B(P<0.05 or P<0.01).Conclusion:CD-SPDP could inhibit DIO and IR,showing that the effect is better than that of CD-SP,and its mechanism is related to promotion of LP and AN secretion and elevation of serum NPY.
Objective: To observe the difference in fatty degree, glucose-lipid disorder and adipose-hormones between diet induced obesity (DIO) rats and diet induced obesity resistance (DIO-R) rats, and to explore the effect and acting mechanism of Chinese drugs for strengthening Pi (spleen, CD-SP) and those for both strengthening Pi and dissolving phlegm (CD-SPDP) in inhibiting obesity. Methods: Excepting eight rats allocated in the blank control group, the other 54 rats were fed with high-lipid forage for 12 weeks to establish models of obesity. Finally, 30 DIO rats and 8 DIO-R rats (shown by their body weight) were obtained. The DIO rats were divided into three groups, which were given gastric perfusion, respectively, with normal saline (Group A), CD-SP (Group B), and CD-SPDP (Group C) .Fourtheen weeks later, the animals’ body weight (BW), length (BL), blood levels of fasting insulin (Fln), fasting glucose (FBG), triglyceride (TG), cholesterol (TC), leptin (LP), neuropeptide Y (NPY), C-reactive protein insulin resistance index (IRI) was calculated, and the degree of obesity and lipid content in abdominal cavity of rats were estimated. More over, the levels of LP, CRP , TNF-α, AN and RS in homogenate of rats’ adipose tissues (ATH) were determined. Results: After 12 weeks of high-lipid diet, the BW of DIO rats were higher than that of normal or DIO-R rats. After (P <0.01); serum levels of TNF-α, LP and AN were lower, NPY was higher in 14-week continuous high-lipid diet feeding, in DIO rats, BW, lipid coefficient , while the ATH levels of LP and AN were lower and TNF-α was higher in DIO rats than in DIO-R rats (P <0.05 or P <0.01); blood levels of FBG and lipids in DIO rats showed an increasing trend but was Significant was found in serum levels of CRP and RS due to the overly high data dispersion. Comparisons of the 3 DIO groups showed that BW, body weight index (BWI), LC a nd IRIwere significantly lowered after treatment (P <0.01) in Group C, while these indexes were not significantly different between Group A and B; the serum levels of TNF-α, LP, and AN increased, NPY decreased in Group B and C, ATH Levels of LP and AN increased, and TNF-αdecreased in the two groups; but NPY, LP, and AN in blood and ATH were higher in Group C than those in Group B (P <0.05 or P <0.01) .Conclusion: CD -SPDP could inhibit DIO and IR, showing that the effect is better than that of CD-SP, and its mechanism is related to promotion of LP and AN secretion and elevation of serum NPY.