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目的:观察CXCR4在结直肠癌及肝和区域淋巴结转移灶中的表达,分析其与结直肠癌临床病理因素的关系。方法:应用SP免疫组化方法检测48例结直肠癌组织、48例癌旁正常组织、15例肝转移灶和23例区域淋巴结转移灶中CX-CR4的表达。结果:48例癌组织中CXCR4表达占95.83%(46/48),弱阳性表达占16.67%(8/48),阳性表达占29.17%(14/48),强阳性表达占50.00%(24/48);48例癌旁正常组织中CXCR4的表达率为16.67%(8/48),均为弱阳性表达。CX-CR4在结直肠癌组织中的表达率(95.83%)明显高于癌旁正常组织(16.67%),χ2=72.38,P<0.01。在伴有肝和区域淋巴结转移的病例中,15例可切除肝转移标本中CXCR4的表达率为100.00%(15/15),其中阳性表达率为20.00%(3/15),强阳性表达率为80%(12/15);23例淋巴结转移标本中CXCR4的表达率为100.00%(23/23),阳性表达率为26.09%(6/23),强阳性表达率为73.91%(17/23)。CXCR4在转移灶中的表达率(100.00%,100.00%)与原发灶中的表达率(95.83%)差异无统计学意义,χ2=1.62,P>0.05;CXCR4高表达与结直肠癌患者的年龄、性别、部位、组织学类型及大体类型无关,P>0.05;而与肝(r=0.43,P<0.05)和区域淋巴结转移(r=0.53,P<0.05)呈正相关。结论:CXCR4在结直肠癌中的表达与肝和区域淋巴结转移呈正相关,检测癌组织CXCR4的表达有助于对结直肠癌患者预后的分析。
OBJECTIVE: To observe the expression of CXCR4 in colorectal cancer, liver and regional lymph node metastasis, and to analyze its relationship with clinicopathological factors of colorectal cancer. Methods: The expression of CXCR4 in 48 colorectal cancer tissues, 48 adjacent normal tissues, 15 liver metastases and 23 regional lymph node metastases were detected by SP immunohistochemistry. Results: The expression of CXCR4 in 48 cases of cancerous tissue was 95.83% (46/48), the weak positive expression was 16.67% (8/48), the positive expression was 29.17% (14/48), the strong positive expression was 50.00% (24 / 48). The expression of CXCR4 in 48 adjacent normal tissues was 16.67% (8/48), both of which were weakly positive. The expression rate of CXCR4 in colorectal cancer tissues was significantly higher than that in normal tissues (95.83% vs 16.67%, χ2 = 72.38, P <0.01). Among cases with hepatic and regional lymph node metastases, the expression rate of CXCR4 in 15 cases of resectable liver metastasis was 100.00% (15/15), and the positive rate of CXCR4 was 20.00% (3/15). The strong positive rate (23/23), the positive rate was 26.09% (6/23), the strong positive rate was 73.91% (17 / twenty three). The expression rate of CXCR4 in metastasis (100.00%, 100.00%) was not significantly different from that in primary tumor (95.83%) (χ2 = 1.62, P> 0.05). CXCR4 overexpression was associated with colorectal cancer P> 0.05), but not with age, gender, location, histological type and gross type (P> 0.05), but positively correlated with liver (r = 0.43, P <0.05) and regional lymph node metastasis (r = 0.53, P <0.05). Conclusion: The expression of CXCR4 in colorectal cancer is positively correlated with the metastasis of liver and lymph nodes. Detecting the expression of CXCR4 in cancerous tissue is helpful to the prognosis of colorectal cancer.