论文部分内容阅读
目的:观察p38MAPK信号转导通路在17β-雌二醇和雷洛昔芬促成骨细胞增殖和分化过程中的作用。方法:取第一继代BALB/c小鼠头盖骨成骨细胞,药物刺激组分别加入不同浓度17β-雌二醇或雷洛昔芬;含阻断剂组预先添加不同浓度的SB202190(p38MAPK的阻断剂)阻断信号转导通路,再加17β-雌二醇或雷洛昔芬,作用72h后用MTT法与PNPP法测定细胞的增殖能力和碱性磷酸酶活性。结果:加入雌激素或雷洛昔芬后,成骨细胞的增殖和分化明显增强,与空白对照组比较差异具有显著性意义(P<0·05);阻断p38MAPK信号转导通路后,细胞增殖分化受到明显抑制,与未阻断组比较差异具有非常显著性意义(P<0.01)。结论:p38MAPK在17β-雌二醇和雷洛昔芬诱导的小鼠成骨细胞的増殖和分化过程中发挥重要作用。
AIM: To investigate the role of p38 MAPK signal transduction pathway in the proliferation and differentiation of osteoblasts induced by 17β-estradiol and raloxifene. Methods: The osteoblasts of the first generation of BALB / c mice were taken into account. The drug-stimulated groups were given different concentrations of 17β-estradiol or raloxifene respectively. Different concentrations of SB202190 Disruptors) block the signal transduction pathway, plus 17β-estradiol or raloxifene 72h after treatment with MTT assay and PNPP assay of cell proliferation and alkaline phosphatase activity. Results: The proliferation and differentiation of osteoblasts were significantly increased after the addition of estrogen or raloxifene compared with the blank control group (P <0.05). After blocking the p38 MAPK signal transduction pathway, the cells Proliferation and differentiation were significantly inhibited, compared with the non-blocking group has a significant difference (P <0.01). CONCLUSION: p38MAPK plays an important role in the proliferation and differentiation of mouse osteoblasts induced by 17β-estradiol and raloxifene.