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家畜精子染色体直接分析技术一直为有关科学家所关注,因其有利于性别控制的研究及实际应用。1978年Rudak等首次用金黄仓鼠卵与人精子进行体外受精获得了人精子过倍染色体,以后Martin等、Brandriff等以及Kamiguchi等也相继成功。本研究旨在利用体外种间受精技术,探索如何区分荷X或Y精子及其自然比率。作者将冻存的精液标本融化,以含0.3%牛血清白蛋白的改良BWW液(pH7.2)洗涤,去除冻存保护液,再用精子爬高的方法收集活动的精子。
Direct analysis of livestock sperm chromosome technology has been concerned by scientists, because it is conducive to gender control research and practical application. In 1978, Rudak et al. Obtained for the first time in vitro fertilization of golden hamster eggs and human spermatozoa to obtain human sperm multiple chromosomes. Later Martin et al., Brandriff et al. And Kamiguchi et al. The aim of this study was to explore how to distinguish between Dutch or Y sperm and their natural ratio by using in vitro fertilization techniques. The authors thaw frozen semen samples, wash them with a modified BWW solution (pH 7.2) containing 0.3% bovine serum albumin (BSA), remove the cryoprotectant solution and collect the sperm by sperm climbing.