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Objective:To investigate the susceptibility to temephos.permethrin and deltamethrin of Aedes aegypti Ae.aegypti),collected from areas with high incidence of dengue hemorrhagic fever cases in Phitsanulok Province.Thailand.Methods:The F1 progenies of Ae.aegypti colony,originated from five sub-districts including Aranyik.Hua Ro,Nai Muang.Ban Krang and Tha Pho,were used in the bioassays following the procedures of World Heath Organization.For larval bioassay.the late third or early fourth-instar lanae were tested with different concentrations of temephos.For adult bioassay.the females were exposed to 0.75%permethrin or 0.05%deltamethrin.LC_(50)value and mortality rate were analyzed to compare the insecticide susceptibility of the larvae and the adults in each area,respectively.Results:The LC_(50) value of temephos for the larvae from Aranyik.Hua Ro.Nai Muang,Ban Krang and Tha Pho sub-districts was 0.017.0.017.0.026.0.061.and 0.113 ppm,respectively.For permethrin,the highest morlalitv rate(86.84%) was found in the mosquitoes from Aranyik but the others were more resistant with the lower morlality rates(16.00-42.67%).The adult morlality rates after exposing to dellaiiielhrin were higher(82.34-98.67%) in all areas.Conclusions:Ae.acgypti larvae were still susceptible to temephos.Conversely,most tested adults tended to resist the penmethrin and deltamethrin.
Objective: To investigate the susceptibility to temephos.permethrin and deltamethrin of Aedes aegypti Ae. Aegypti), collected from areas with high incidence of dengue hemorrhagic fever cases in Phitsanulok Province. Thailand. Methods: The F1 progenies of Ae. Aegypti colony, originated from five sub-districts including Aranyik. Hua Ro, Nai Muang.Ban Krang and Tha Pho, were used in the bioassays following the procedures of World Heath Organization. For larval bioassay. the late third or early fourth-instar lanae were tested with different concentrations of temephos. For adult bioassay. the females were exposed to 0.75% permethrin or 0.05% deltamethrin.LC - (50) value and mortality rate were analyzed to compare the insecticide susceptibility of the larvae and the adults in each area, respectively. Results: The LC_ (50) value of temephos for the larvae from Aranyik.Hua Ro.Nai Muang, Ban Krang and Tha Pho sub-districts was 0.017.0.017.0.026.0.061.and 0.113 ppm, respectively.For permethrin, the highest morlalitv rate ( 86.84%) was found in the mosquitoes from Aranyik but the others were more resistant with the lower morlality rates (16.00-42.67%). The adult morlality rates after exposing to dellaiiielhrin were higher (82.34-98.67%) in all areas. Conclusions: Ae.acgypti larvae were still susceptible to temephos.Conversely, most tested adults tended to resist the penmethrin and deltamethrin.