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目的:评价重组免疫毒素hIL2-Luffin P1联合维A酸乙酯对皮肤T淋巴瘤细胞(Hut-78)增殖、凋亡及细胞周期的影响。方法:将不同浓度hIL2-Luffin P1分别联合10~(-7)mol/L维A酸乙酯处理Hut-78细胞,用MTT法检测细胞抑制率,流式细胞仪检测细胞凋亡及细胞周期。结果:用浓度为0.5、1、10、20、30、40 μg/mL的hIL2-Luffin P1分别联合10~(-7)mol/L的芳维A酸乙酯作用Hut-78细胞48h,对Hut-78细胞的抑制率分别为21.33±1.65、28.30±2.42、40.58±1.69、51.57±2.54、63.15 4-2.41、72.45±1.76;用30 μg/mL的hIL2-Luffin P1联合10~(-7)mol/L芳维A酸乙酯分别作用于Hut-78细胞12、24、36、48 h,对Hut-78细胞的抑制率为26.96±1.91、38.05±1.64、51.22±0.57、64.30 4-2.19、72.48±2.23。经浓度为1、30μg/mL的hIL2-Lufifin P1分别联合10~(-7)mol/L的维A酸乙酯作用Hut-78细胞48h,细胞的凋亡率分别为15.94 ±1.66和34.89±2.11。经30μg/mL的hIL-2-Luffin P1联合10~(-7)mol/L的维A酸乙酯作用Hut-78细胞48 h,主要表现为G_1期细胞比例增加和S期减少。结论:hIL2-Luf-fin P1联合维A酸乙酯能够抑制Hut-78细胞的增殖及凋亡。
OBJECTIVE: To evaluate the effect of recombinant immunotoxin hIL2-Luffin P1 combined with retinoic acid ethyl ester on the proliferation, apoptosis and cell cycle of cutaneous T lymphoma cells (Hut-78). Methods: Hut-78 cells were treated with different concentrations of hIL2-Luffin P1 and 10 -7 mol / L retinoic acid ethyl ester respectively. The inhibition rates of cells were determined by MTT assay. The apoptosis and cell cycle were detected by flow cytometry . Results: Hut-78 cells were treated with hIL2-Luffin P1 at concentration of 0.5, 1, 10, 20, 30 and 40 μg / mL for 48 h with 10 -7 mol / The inhibitory rates of Hut-78 cells were 21.33 ± 1.65, 28.30 ± 2.42, 40.58 ± 1.69, 51.57 ± 2.54, 63.15 4-2.41, 72.45 ± 1.76, respectively. Hut-78 cells were treated with 30 μg / mL hIL2- ) mol / L of aryl retinoic acid ethyl ester on Hut-78 cells for 12,24,36,48 h, the inhibition rate of Hut-78 cells were 26.96 ± 1.91,38.05 ± 1.64,51.22 ± 0.57,64.30 4- 2.19, 72.48 ± 2.23. Hut-78 cells were treated with hIL2-Lufifin P1 at concentration of 1 and 30μg / mL for 48h respectively with 10 -7 mol / L retinoic acid ethyl ester for 15 h, respectively. The apoptotic rates of Hut-78 cells were 15.94 ± 1.66 and 34.89 ± 2.11. Hut-78 cells were treated with 30μg / mL hIL-2-Luffin P1 combined with 10 -7 mol / L retinoic acid ethyl ester for 48 h, which showed that the proportion of cells in G 1 phase was increased and the S phase was decreased. Conclusion: hIL2-Luf-fin P1 combined with retinoic acid ethyl ester can inhibit Hut-78 cell proliferation and apoptosis.