论文部分内容阅读
目的研究微囊蛋白-1(caveolin-1)及增殖细胞核抗原(PCNA)在脑胶质瘤中的表达情况,探讨两者在胶质瘤的发生发展中的作用及相互关系。方法选取80例胶质瘤患者的手术切除标本,采用免疫组化(SP)法检测Caveolin-1和PCNA的表达情况,8例正常脑组织作为对照。结果 Caveolin-1和PCNA在胶质瘤中的阳性表达率分别为48.75%和68.75%。Caveolin-1在高级别胶质瘤(Ⅲ～Ⅳ级)中的OD值为(0.112±0.008),在低级别胶质瘤(Ⅰ～Ⅱ级)中的OD值为(0.135±0.016),两者差异有统计学意义(P<0.05);PCNA在高级别胶质瘤(Ⅲ～Ⅳ级)中的OD值为(0.130±0.010),在低级别胶质瘤(Ⅰ～Ⅱ级)中的OD值为(0.118±0.010),两者差异有统计学意义(P<0.05)。Caveolin-1与PCNA在胶质瘤中的表达呈负相关性(r=-0.368,P<0.01)。结论 Caveolin-1在胶质瘤中表现为一个抑癌基因,它的表达对肿瘤细胞增殖起抑制作用,有可能为胶质瘤的基因治疗提供了一个新的靶点;PCNA是反映胶质瘤细胞增殖的可靠指标,两者均可作为临床判断胶质瘤患者恶性程度及预后的指标。
Objective To investigate the expression of caveolin-1 and proliferating cell nuclear antigen (PCNA) in gliomas and to explore the roles and relationship between caveolin-1 and proliferating cell nuclear antigen (PCNA) in gliomas. Methods Totally 80 patients with glioma were selected for surgical resection. The expressions of Caveolin-1 and PCNA were detected by immunohistochemistry (SP). Eight normal brain tissues were used as control. Results The positive rates of Caveolin-1 and PCNA in gliomas were 48.75% and 68.75%, respectively. The OD value of Caveolin-1 in high grade gliomas (grade Ⅲ ~ Ⅳ) was (0.112 ± 0.008) and in low grade gliomas (grade Ⅰ ~ Ⅱ) was (0.135 ± 0.016) (P <0.05). The OD value of PCNA in high grade gliomas (grade Ⅲ ~ Ⅳ) was (0.130 ± 0.010), while in low grade gliomas (grade Ⅰ ~ Ⅱ), the difference was statistically significant OD value was (0.118 ± 0.010), the difference was statistically significant (P <0.05). The expression of Caveolin-1 and PCNA in glioma was negatively correlated (r = -0.368, P <0.01). Conclusions Caveolin-1 is a tumor suppressor gene in glioma. Its expression inhibits the proliferation of tumor cells and may provide a new target for the gene therapy of gliomas. PCNA is a tumor- A reliable indicator of cell proliferation, both of which can be used as clinical indicators to determine the degree of malignancy and prognosis of glioma patients.