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目的探讨子宫颈病变中c-myc与hMLH1和HPV16表达的关系。方法应用免疫组化SP法检测c-myc与hM-LH1在慢性子宫颈炎、子宫颈上皮内瘤变(cervical intraepi-thelial neoplasia,CIN)和子宫颈癌患者中的表达水平;PCR技术检测相应标本中HPV16感染的情况。结果 c-myc在慢性子宫颈炎、CIN、子宫颈癌中的阳性率分别为26.7%(8/30))、50%(30/60)、69.2%(36/52),差异有统计学意义(P<0.05),hMLH1阳性率依次为66.7%(20/30)、56.7%(34/60)、30.7%(16/52),差异有统计学意义(P<0.05)。在子宫颈癌组织中,c-myc蛋白与肿瘤分化程度、临床分期以及有无淋巴结转移相关;hMLH1蛋白表达下调与子宫颈癌分化程度及是否有淋巴结转移密切相关,表达差异均有统计学意义(P<0.05);HPV16与c-myc蛋白表达呈正相关;与hM-LH1蛋白表达呈负相关。结论 HPV16可能是通过影响c-myc与hMLH1蛋白表达而在子宫颈癌的发生、发展中发挥作用。
Objective To investigate the relationship between c-myc and the expression of hMLH1 and HPV16 in cervical lesions. Methods The expression of c-myc and hM-LH1 in patients with chronic cervicitis and cervical intraepithelial neoplasia (CIN) and cervical cancer was detected by immunohistochemical SP method. The levels of c-myc and hM- HPV16 infection. Results The positive rates of c-myc in chronic cervicitis, CIN and cervical cancer were 26.7% (8/30), 50% (30/60) and 69.2% (36/52) respectively, the difference was statistically significant (P <0.05). The positive rates of hMLH1 were 66.7% (20/30), 56.7% (34/60) and 30.7% (16/52), respectively. The difference was statistically significant (P <0.05). In cervical cancer, c-myc protein was correlated with the degree of tumor differentiation, clinical stage and lymph node metastasis. The down-regulation of hMLH1 protein was closely related to the differentiation of cervical cancer and lymph node metastasis, and the differences were statistically significant (P <0.05). There was a positive correlation between HPV16 and c-myc protein expression and a negative correlation with hM-LH1 protein expression. Conclusion HPV16 may play an important role in the occurrence and development of cervical cancer by affecting the expression of c-myc and hMLH1.