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目的建立玄蓝咽喉炎颗粒中黄芩苷和牛蒡苷的测定方法。方法采用HPLC法,色谱柱:SHIMADZU VP-ODS(150 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸溶液(38∶62);流速1.0 ml·min-1;柱温:30℃;检测波长:280 nm。结果黄芩苷、牛蒡苷在25.9~259.0、5.09~50.9μg·ml-1范围内有良好的线性关系;黄芩苷的平均回收率为99.78%,RSD为2.28%;牛蒡苷的平均回收率100.45%,RSD为2.26%。结论本方法简便可行,重复性好,可用于玄蓝咽喉炎颗粒的质量控制。
Objective To establish a method for the determination of baicalin and arctiin in Xuan Lan pharyngitis granules. Methods The HPLC method was used. The chromatographic column was SHIMADZU VP-ODS (150 mm × 4.6 mm, 5 μm). The mobile phase was methanol-0.1% phosphoric acid solution (38:62) Detection wavelength: 280 nm. Results Baicalin and arctiin had a good linear relationship in the range of 25.9-259.0 and 5.09-50.9μg · ml-1. The average recovery of baicalin was 99.78%, RSD was 2.28%. The average recovery of arctiin was 100.45% , RSD was 2.26%. Conclusion This method is simple and feasible, reproducible, and can be used for quality control of Xuan Lanyan laryngitis particles.