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Objective:To investigate the effect of IL-6 gene transfe r into human cord blood hematopoietic stem cells on the production of megakaryocy t ic progenitors.Methods:IL-6 gene was transfected into human c o rd blood CD34+ cells using a retrovirus vector with the aid of recombinant fibro nectin fragments in the presence of a cocktail of cytokines (SCF,IL-6,sIL-6R,F L,and TPO).Colony-forming units-megakaryocyte (CFU-MK) assays were performed as IL-6 gene transduced CD34+ cells were incubated alone or in combination with I L -3 or sIL-6R, controlled with neoR gene transduced CD34+ cells.Result s :IL-6 alone or sIL-6R alone stimulated few CFU-MK colonies,the addit ion of sIL-6R to IL-6 gene transduced CD34+ cells significantly enhanced the prod uction of CFU-MK colonies.IL-6 gene transduced CD34+ cells showed a modest syn er gistic effect with IL-3.Conclusion:These results suggest that IL-6 gene transfer may protect patients from chemotherapy-induced thrombocytop en ia.
Objective: To investigate the effect of IL-6 gene transfer r into human cord blood hematopoietic stem cells on the production of megakaryocyt ic progenitors. Methods: IL-6 gene was transfected into human co rd blood CD34 + cells using a retrovirus vector with the aid of recombinant fibro nectin fragments in the presence of a cocktail of cytokines (SCF, IL-6, sIL-6R, FL, and TPO). Colony-forming units-megakaryocyte transduced CD34 + cells were incubated alone or in combination with IL-3 or sIL-6R, controlled with neoR gene transduced CD34 + cells. Result s: IL-6 alone or sIL-6R alone stimulated few CFU-MK colonies, the additon of sIL -6R to IL-6 gene transduced CD34 + cells significantly enhanced the prod uction of CFU-MK colonies. IL-6 gene transduced CD34 + cells showed a modest syn er gistic effect with IL-3.Conclusion: These results suggest that IL-6 gene transfer may protect patients from chemotherapy-induced thrombocytoposis ia.