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目的:探讨表皮生长因子受体(EGFR)特异性抑制剂西妥昔单抗(C225)以及与化疗药物奈达铂(NDP)联合应用对宫颈癌He La细胞的杀伤作用,并分析其作用机制。方法:培养宫颈癌He La细胞;实验分为C225单药组(10、20、40、60、80和160 mg/L)、NDP单药组(1、5、10、20、40和60 mg/L)、C225+NDP联合用药组(C225 40 mg/L分别联合NDP各浓度),每个浓度作用He La细胞后分别培养24、48和72 h后采用MTT法检测细胞抑制率;采用流式细胞术检测细胞凋亡和周期。结果:C225单药不能明显抑制He La细胞增殖;NDP单药对hela细胞有明显抑制作用,C225联合NDP的细胞抑制率明显高于对应的C225或NDP单药(P<0.05)。NDP和C225单独应用均有促进细胞凋亡的作用,联合用药后凋亡率均大于对应的NDP或C225单药(P均<0.05)。NDP或C225单药时,G0-G1期细胞百分比较对照组增加,S期较对照组减少,两药联合后,G0-G1期细胞百分比明显增加(P<0.05),S期细胞百分比明显降低(P<0.05),细胞增殖指数(PI)也逐渐降低(P<0.05)。结论:C225可增强hela细胞对NDP的敏感性,增加细胞凋亡率,G0-G1期阻滞作用增强。
OBJECTIVE: To investigate the killing effect of cetuximab (C225), a specific inhibitor of epidermal growth factor receptor (EGFR), and cervical cancer He La cells in combination with NDP (chemotherapy drug), and to investigate its mechanism of action . Methods: Cervical HeLa cells were cultured. The experimental groups were divided into C225 monotherapy group (10,20,40,60,80 and 160 mg / L), NDP monotherapy group (1,5,10,20,40 and 60 mg / L) and C225 + NDP combination group (C225 40 mg / L, respectively, with NDP concentration). After each concentration of Hela cells were cultured for 24, 48 and 72 h respectively, the cell inhibition rate was measured by MTT assay. Cytometry to detect apoptosis and cycle. Results: C225 alone did not significantly inhibit the proliferation of HeLa cells. NDP alone had a significant inhibitory effect on hela cells. The inhibition rate of C225 combined with NDP was significantly higher than that of C225 or NDP alone (P <0.05). Both NDP and C225 alone had the effect of promoting apoptosis. The apoptotic rate of the combination group was higher than that of NDP or C225 alone (all P <0.05). NDP or C225 alone increased the percentage of cells in G0-G1 phase and decreased in S phase compared with control group. The percentage of cells in G0-G1 phase increased significantly (P <0.05) and the percentage of cells in S phase decreased significantly (P <0.05), and the cell proliferation index (PI) also decreased gradually (P <0.05). Conclusion: C225 can enhance the sensitivity of hela cells to NDP, increase the apoptosis rate and enhance the arrest of G0-G1 phase.