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Some wild species of Oryza possess agronomically useful traits such as disease/insect resis tance and salt tolerance. However, the use of wild Oryza species for rice improvement has been hampered by the sexual incompatibility or sterility of hybrids obtained by conventional methods. We have used protoplast fusion as a method to incorporate useful trait of wild O ryza species into cultivated rice and obtained somatic hybrid plants between O.satlva and O.officinalis and their progenies. Isozyme analysis is a method to confirm somatic hybrids and to determine the nuclear constitution of somatic hybrids. The esterase and peroxidase isozyme analysis in progenies of somatic hybrids were herein reported. Two breeding lines PF52 and PF79 were F_2 lines developed form somatic hybrids of O.sativa L.cv. 02428 and O.officinalis accesion. O.sativa L.cv. 02428 and O.officinalis were used as control. Fresh leaves from plants growing in greenhouse were homogenized with extraction
Some, wild species of Oryza possess agronomically useful traits such as disease / insect resis tance and salt tolerance. However, the use of wild Oryza species for rice improvement has been hampered by the sexual incompatibility or sterility of hybrids obtained by conventional methods. We have used protoplast fusion as a method to incorporate trait of wild O ryza species into cultivated rice and obtain somatic hybrid plants between O. satlva and O. officinalis and their progenies. Isozyme analysis is a method to confirm somatic hybrids and to determine the nuclear constitution of somatic hybrids. The esterase and peroxidase isozyme analysis in progenies of somatic hybrids were compared reported. Two breeding lines PF52 and PF79 were F_2 lines developed form somatic hybrids of O.sativa L.cv. 02428 and O. officinalis accesion. O.sativa L .cv. 02428 and O. officinalis were used as control. Fresh leaves from plants growing in greenhouse were homogenized with extraction