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为了建立一种稳定而有效的细胞杂交方法,作者用神经氨酸苷酶对B细胞和骨髓瘤细胞进行处理,以去掉细胞表面的唾液酸—因为唾液酸具有阻止细胞彼此靠近的作用一创造一种细胞间更紧密联系。这种处理对人一鼠异种杂交瘤的建立同样重要。 具体方法是将脾细胞和骨髓瘤细胞Ps X_(63)Ag8.653分为2组,处理一组,另一组对照。处理组的细胞分别悬于含2单位神经氨酸苷酶的10ml RPMI-1640完全培养液中,
In order to establish a stable and effective cell hybridization method, the authors used neuramididases to treat B cells and myeloma cells to remove sialic acid from the cell surface - because sialic acid has the effect of preventing cells from getting close to each other. The cells are more closely linked. This treatment is equally important for the establishment of human-mouse hybridomas. The specific method is to divide the spleen cells and myeloma cells Ps X_(63)Ag8.653 into 2 groups to treat one group and the other to control. The cells of the treatment group were separately suspended in 10 ml RPMI-1640 complete medium containing 2 units of neuraminidase,